顺式作用RNA序列控制鼠白血病病毒中的gag-pol翻译通读。
cis Acting RNA sequences control the gag-pol translation readthrough in murine leukemia virus.
作者信息
Honigman A, Wolf D, Yaish S, Falk H, Panet A
机构信息
Department of Molecular Genetics and Virology, Hebrew University, Hadassah Medical School, Jerusalem, Israel.
出版信息
Virology. 1991 Jul;183(1):313-9. doi: 10.1016/0042-6822(91)90144-z.
Abstract
The pol gene of the Moloney murine leukemia virus (M-MuLV) is expressed as a Gag-Pol fusion protein through an in-frame suppression of the UAG termination codon located between the two genes. The role of nucleotide context in suppression was investigated, in a rabbit reticulocyte lysate translation system, using site-directed mutagenesis. The results indicate that the translational readthrough is mediated by at least 50 bases long RNA sequence located 3' to the gag UAG termination codon. Within this sequence a short purine-rich sequence adjacent to the amber codon, highly conserved among different retroviruses, appears essential for M-MuLV suppression. Two alternative putative stem and loop like RNA structures can be drawn at the gag-pol junction, one abutting the gag UAG codon, and the second downstream to it. None of these structures appears to be important to the suppression process.
摘要
莫洛尼鼠白血病病毒（M-MuLV）的pol基因通过对位于两个基因之间的UAG终止密码子进行框内抑制，以Gag-Pol融合蛋白的形式表达。利用定点诱变技术，在兔网织红细胞裂解物翻译系统中研究了核苷酸上下文在抑制过程中的作用。结果表明，翻译通读由位于gag UAG终止密码子3'端至少50个碱基长的RNA序列介导。在该序列中，紧邻琥珀密码子的一段短的富含嘌呤的序列，在不同逆转录病毒中高度保守，似乎对M-MuLV的抑制至关重要。在gag-pol连接处可以画出两种不同的假定茎环样RNA结构，一种邻接gag UAG密码子，另一种在其下游。这些结构似乎对抑制过程都不重要。
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