Stohwasser R, Raab K, Darai G, Bautz E K
ZMBH, Heidelberg, Germany.
Virology. 1991 Jul;183(1):386-91. doi: 10.1016/0042-6822(91)90152-2.
The L RNA segment of the nephropathia epidemica virus (NEV) strain Hällnäs B1 was characterized by molecular cloning of the corresponding cDNA and subsequent determination of the DNA nucleotide sequence. The L RNA segment is 6550 nucleotides long with complementarity of 20 bases at the 3' and 5' termini. The viral messenger sense RNA contains one major open reading frame (ORF) with a coding capacity of 2156 amino acid residues encoding a protein with a calculated molecular weight of 246 kDa and an IEP of pH 7.4. Comparison of the deduced amino acid sequences from NEV hantavirus and Bunyamwera virus (BWV) L segment messenger sense RNAs, revealed a high degree of diversity (overall amino acid identity, 17%). However, three clusters of 30-40% amino acid identity were detected. One of these domains, containing an Asp-Asp motif found in many RNA polymerases, also shares amino acid sequence homology with the PB1 polymerase component of influenza type A. These results indicate that the L RNA segment of the NEV codes for the viral RNA-dependent RNA polymerase. The data presented here complete our previous studies on the characterization of the NEV genome by cDNA sequencing of the viral M and S RNA segments.
通过对肾病综合征出血热病毒（NEV）哈尔纳斯B1株L RNA片段相应cDNA进行分子克隆及随后测定DNA核苷酸序列，对其进行了特性分析。L RNA片段长6550个核苷酸，3'和5'末端有20个碱基的互补性。病毒信使链RNA包含一个主要开放阅读框（ORF），编码能力为2156个氨基酸残基，编码一种计算分子量为246 kDa、等电点为pH 7.4的蛋白质。对NEV汉坦病毒和布尼亚姆韦拉病毒（BWV）L片段信使链RNA推导的氨基酸序列进行比较，发现高度多样性（总体氨基酸同一性为17%）。然而，检测到三个氨基酸同一性为30 - 40%的簇。其中一个结构域含有许多RNA聚合酶中发现的天冬氨酸-天冬氨酸基序，也与甲型流感病毒的PB1聚合酶成分具有氨基酸序列同源性。这些结果表明，NEV的L RNA片段编码病毒RNA依赖的RNA聚合酶。本文呈现的数据完善了我们之前通过对病毒M和S RNA片段进行cDNA测序对NEV基因组进行特性分析的研究。
