Antic D, Lim B U, Kang C Y
Department of Microbiology and Immunology, University of Ottawa, Faculty of Medicine, Ontario, Canada.
Virus Res. 1991 Mar;19(1):47-58. doi: 10.1016/0168-1702(91)90093-b.
The genomic M segment of Seoul 80-39 virus was characterized by cloning and nucleotide sequence analysis. The virion M RNA segment is 3651 nucleotides long with the 3' and 5' terminal sequences inversely complementary for 20 bases. A single open reading frame was detected in the viral complementary-sense RNA which can encode a polypeptide of 1133 amino acids. The Seoul 80-39 virus M segment was compared with the M segments of related viruses, Hantaan 76-118, Hallnas B1 and Sapporo Rat (SR-11) virus. Our results demonstrate a significant similarity between M RNA segments of the Seoul 80-39, Hantaan 76-118, Hallnas B1 and SR-11 viruses. The degree of conservation of both nucleic acid and protein sequences between these viruses reveals a close evolutionary relationship. Furthermore, it is evident that the serotypic profile of hantaviruses is determined by the rodent host species from which the virus was isolated and not by the geographical area.
通过克隆和核苷酸序列分析对汉城80 - 39病毒的基因组M片段进行了表征。病毒粒子的M RNA片段长3651个核苷酸，其3'和5'末端序列有20个碱基的反向互补。在病毒负链RNA中检测到一个单一的开放阅读框，它可以编码一个1133个氨基酸的多肽。将汉城80 - 39病毒的M片段与相关病毒汉滩76 - 118、霍尔纳斯B1和札幌大鼠（SR - 11）病毒的M片段进行了比较。我们的结果表明，汉城80 - 39、汉滩76 - 118、霍尔纳斯B1和SR - 11病毒的M RNA片段之间存在显著相似性。这些病毒之间核酸和蛋白质序列的保守程度揭示了密切的进化关系。此外，显然汉坦病毒的血清型特征是由分离病毒的啮齿动物宿主物种决定的，而不是由地理区域决定的。
