Department of Anatomy, Key Lab for Biomechanics of Chongqing, Third Military Medical University, Gao Tan Yan Street, Sha Ping Ba District, Chongqing 400038, China.
Colloids Surf B Biointerfaces. 2012 Aug 1;96:29-36. doi: 10.1016/j.colsurfb.2012.03.014. Epub 2012 Mar 29.
Previous studies have shown that piezoelectric materials may be used to prepare bioactive electrically charged surfaces. In the current study, polyurethane/polyvinylidene fluoride (PU/PVDF) scaffolds were prepared by electrospinning. The mechanical property and piezoelectric property of the scaffolds were evaluated. The crystalline phase of PVDF in the scaffolds was characterised by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC). In vitro cell culture was performed to investigate cytocompatibility of the scaffolds. Wound-healing assay, cell-adhesion assay, quantitative RT-PCR and Western blot analyses were performed to investigate piezoelectric effect of the scaffolds on fibroblast activities. Further, the scaffolds were subcutaneously implanted in Sprague-Dawley (SD) rats to investigate their biocompatibility and the piezoelectric effect on fibrosis in vivo. The results indicated that the electrospinning process had changed PVDF crystalline phase from the nonpiezoelectric α phase to the piezoelectric β phase. The fibroblasts cultured on the scaffolds showed normal morphology and proliferation. The fibroblasts cultured on the piezoelectric-excited scaffolds showed enhanced migration, adhesion and secretion. The scaffolds that were subcutaneously implanted in SD rats showed higher fibrosis level due to the piezoelectrical stimulation, which was caused by random animal movements followed by mechanical deformation of the scaffolds. The scaffolds are potential candidates for wound healing applications.
先前的研究表明，压电材料可用于制备具有生物活性的带电表面。本研究通过静电纺丝制备了聚氨酯/聚偏氟乙烯（PU/PVDF）支架。评估了支架的机械性能和压电性能。通过 X 射线衍射（XRD）、傅里叶变换红外光谱（FTIR）和差示扫描量热法（DSC）对支架中 PVDF 的晶相进行了表征。进行体外细胞培养以研究支架的细胞相容性。进行了划痕愈合试验、细胞黏附试验、定量 RT-PCR 和 Western blot 分析，以研究支架对成纤维细胞活性的压电效应。此外，将支架皮下植入 Sprague-Dawley（SD）大鼠体内，以研究其体内的生物相容性和对纤维化的压电效应。结果表明，静电纺丝过程将 PVDF 晶相从非压电的α相转变为压电的β相。在支架上培养的成纤维细胞形态和增殖正常。在压电激发支架上培养的成纤维细胞表现出增强的迁移、黏附和分泌。由于支架的随机动物运动和随后的机械变形，皮下植入 SD 大鼠的支架表现出更高的纤维化水平。该支架是伤口愈合应用的潜在候选材料。
