接受抗血小板治疗的肾移植受者中的网织血小板与血小板反应性

Reticulated platelets and platelet reactivity in renal transplant recipients receiving antiplatelet therapy.

作者信息

Zanazzi M, Cesari F, Rosso G, Farsetti S, Caroti L, Gori A M, Cerini G, Cioni G, Bertoni E, Abbate R, Salvadori M

机构信息

Renal Unit, Department of Medical and Surgical Critical Care, Thrombosis Centre, University of Florence and Careggi University Hospital, Florence, Italy.

出版信息

Transplant Proc. 2010 May;42(4):1156-7. doi: 10.1016/j.transproceed.2010.03.042.

DOI:10.1016/j.transproceed.2010.03.042

PMID:20534248

Abstract

INTRODUCTION

Renal transplant recipients are at increased risk of cardiovascular morbidity and mortality. We assessed platelet reactivity and reticulated platelets (RPs) in 90 recipients, 51 (56.6%) of whom were not receiving acetylsalicylic acid (ASA) therapy (group A) and 39 (43.3%) who were receiving ASA therapy, 100 mg (group B), and in 60 healthy controls (group C).

METHODS

Reticulated platelets were measured using a hematology automated analyzer (XE-2100; Sysmex Corp, Kobe, Japan) and were expressed as the percentage of RPs in the total optical platelet count (immature platelet fraction [IPF]), as the percentage of highly fluorescent RPs, and as the absolute number of RPs (IPF#). Platelet function was assessed using optical aggregometry (platelet aggregation) induced using 1 mmol/L of arachidonic acid, 2 or 10 micromol/L of adenosine diphosphate, or 2 microg/mL of collagen.

RESULTS

Group A demonstrated significantly higher values of RP compared with group B or group C. Group B demonstrated a substantially higher percentage of RPs compared with group C, which was significant only for the IPF parameter. Multiple regression analysis demonstrated that IPF and IPF# were significantly and positively related to collagen-induced platelet aggregation.

CONCLUSION

We documented the presence of higher concentrations of RPs in transplant recipients compared with a control population, and a significant association between RPs and platelet function.

摘要

引言

肾移植受者发生心血管疾病的发病率和死亡率风险增加。我们评估了90名受者的血小板反应性和网织血小板（RP），其中51名（56.6%）未接受乙酰水杨酸（ASA）治疗（A组），39名（43.3%）接受100mg ASA治疗（B组），并与60名健康对照者（C组）进行比较。

方法

使用血液学自动分析仪（XE-2100；日本神户Sysmex公司）测量网织血小板，并表示为网织血小板在总光学血小板计数中的百分比（未成熟血小板分数[IPF]）、高荧光网织血小板的百分比以及网织血小板的绝对数量（IPF#）。使用光学比浊法（血小板聚集）评估血小板功能，诱导剂为1mmol/L花生四烯酸、2或10μmol/L二磷酸腺苷或2μg/mL胶原蛋白。