免疫层析试纸条快速检测人血清中的克氏锥虫。
Rapid detection of Trypanosoma cruzi in human serum by use of an immunochromatographic dipstick test.
机构信息
Department of Infectious & Tropical Diseases, London School of Hygiene & Tropical Medicine, London, United Kingdom.
出版信息
J Clin Microbiol. 2010 Aug;48(8):3003-7. doi: 10.1128/JCM.02474-09. Epub 2010 Jun 9.
Abstract
We evaluated a commercially available immunochromatographic dipstick test to detect Trypanosoma cruzi infection in 366 human serum samples with known serological results from Argentina, Ecuador, Mexico, and Venezuela. One hundred forty-nine of 366 (40.7%) and 171/366 (46.7%) samples tested positive by dipstick and serology, respectively. Dipstick sensitivity was calculated to be 84.8% (range between countries, 77.5 to 95%), and specificity was 97.9% (95.9 to 100%).
摘要
我们评估了一种市售的免疫层析检测试剂盒，用于检测来自阿根廷、厄瓜多尔、墨西哥和委内瑞拉的 366 个人类血清样本中的克氏锥虫感染。分别有 149/366（40.7%）和 171/366（46.7%）的样本通过免疫层析和血清学检测呈阳性。免疫层析的灵敏度计算为 84.8%（各国之间的范围为 77.5%至 95%），特异性为 97.9%（95.9%至 100%）。
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1
[Maternal-fetal transmission of Trypanosoma cruzi in Argentina].[克氏锥虫在阿根廷的母婴传播]
Medicina (B Aires). 2009;69(5):529-35.
2
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J Clin Microbiol. 2009 Dec;47(12):3945-51. doi: 10.1128/JCM.01601-09. Epub 2009 Oct 21.
3
Eliminating Chagas disease: challenges and a roadmap.消除恰加斯病：挑战与路线图。
BMJ. 2009 Apr 14;338:b1283. doi: 10.1136/bmj.b1283.
4
Geographic variation in the sensitivity of recombinant antigen-based rapid tests for chronic Trypanosoma cruzi infection.基于重组抗原的慢性克氏锥虫感染快速检测敏感性的地理差异。
Am J Trop Med Hyg. 2009 Mar;80(3):410-5.
5
[Evaluation of SD BIOLINE Chagas Ab Rapid kit].[SD生物线查加斯抗体快速检测试剂盒评估]
Korean J Lab Med. 2009 Feb;29(1):48-52. doi: 10.3343/kjlm.2009.29.1.48.
6
Chagas disease: state-of-the-art of diagnosis and management.恰加斯病：诊断与管理的最新进展
Cardiol J. 2008;15(6):493-504.
7
8
False-positive results of a rapid K39-based strip test and Chagas disease.基于K39的快速试纸检测的假阳性结果与恰加斯病
Int J Infect Dis. 2009 Mar;13(2):182-5. doi: 10.1016/j.ijid.2008.06.003. Epub 2008 Sep 12.
9
International meeting: new diagnostic tests are urgently needed to treat patients with Chagas disease.国际会议：治疗恰加斯病患者急需新的诊断测试。
Rev Soc Bras Med Trop. 2008 May-Jun;41(3):315-9. doi: 10.1590/s0037-86822008000300020.
10
[A confirmatory diagnosis of antibodies anti-Trypanosoma cruzi in donors referred by blood banks in Venezuela].[委内瑞拉血库转诊供体中抗克氏锥虫抗体的确证诊断]
Invest Clin. 2008 Jun;49(2):141-50.
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