橙皮苷促进 UVB 暴露小鼠表皮中环丁烷嘧啶二聚体的修复。
Hesperidin promotes cyclobutane pyrimidine dimer repair in UVB-exposed mice epidermis.
机构信息
Department of Dermatology, The First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China.
出版信息
Ir J Med Sci. 2011 Sep;180(3):709-14. doi: 10.1007/s11845-010-0503-5. Epub 2010 Jun 11.
PURPOSE
To investigate whether topical application of hesperin affords protection to Balb/C mice epidermis from UVB-induced cyclobutane pyrimidine dimers (CPDs).
METHODS
A DNA damage model of UVB irradiation-induced mice epidermis was established. The immunohistochemical staining and southwestern dot blotting were used for CPDs detection; western blotting was used for P53 detection.
RESULTS
Topical application of hesperidin on Balb/C mice skin significantly decreased the amount of epidermal CPDs 24 and 48 h after 180 mJ/cm(2) of UVB irradiation as compared to untreated mice. UVB-induced p53 expression was more pronounced in hesperidin-treated mice epidermis compared to that of untreated mice.
CONCLUSION
Taken together, these results suggest that topical hesperidin application promotes DNA photo-damage repair. Hesperidin is therefore a promising protective substance against UVB radiation.
目的
研究橙皮苷是否能保护 Balb/C 小鼠表皮免受 UVB 诱导的环丁烷嘧啶二聚体（CPD）的损伤。
方法
建立 UVB 照射诱导的小鼠表皮 DNA 损伤模型。采用免疫组化染色和西南点印迹法检测 CPD；采用 Western blot 法检测 P53。
结果
与未处理的小鼠相比，橙皮苷局部应用于 Balb/C 小鼠皮肤后，在 180 mJ/cm(2)UVB 照射后 24 和 48 小时，表皮 CPD 的量明显减少。与未处理的小鼠相比，橙皮苷处理的小鼠表皮中 UVB 诱导的 p53 表达更为明显。
结论
综上所述，这些结果表明，局部应用橙皮苷可促进 DNA 光损伤修复。因此，橙皮苷是一种有前途的抗 UVB 辐射的保护物质。
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