Center for Stem Cell Biology and Tissue Engineering, Sun Yat-Sen University, Guangzhou 510080, P. R. China.
J Biomater Sci Polym Ed. 2010;21(12):1653-67. doi: 10.1163/092050609X12531835454314. Epub 2010 Jun 9.
Recently, tissues adjacent to the wound sites are regarded as a promising therapeutic cell source for curing and repairing purpose. Specifically, therapeutic stem cells have been identified in synovial tissue, a tissue adjacent to articular cartilage. The purpose of this study was to explore therapeutic chondrogenesis with rabbit synovium-derived mesenchymal stem cells (SMSCs) encapsulated in photopolymerized hydrogels. A non-degradable poly(ethylene glycol) diacrylate (PEGDA)-based hydrogel and biodegradable phosphoester-poly(ethylene glycol) (PhosPEG)-based hydrogel were both applied as 3-D scaffolds mediating SMSC chondrogenesis in vitro. The viability of SMSCs in both hydrogels was assessed by fluorescent Live/Dead assay and WST-1 assay. Levels of genes and proteins specific to SMSC chondrogenesis were evaluated by real-time RT-PCR, biochemical analysis and immunohistochemical analysis, respectively. The results demonstrated that SMSCs continue to have a high viability when encapsulated in the hydrogel. By treatment with transforming growth factor (TGF)-beta1 or TGF-beta3, positive SMSC chondrogenesis was successfully achieved in both gels, with the best outcome in the PEGDA system. It can be concluded that both PEGDA and PhosPEG hydrogels are appropriate cell-delivery vehicles for SMSC chondrogenesis. Especially as a biodegradable material, PhosPEG hydrogel displayed great potentials in future applications for articular cartilage regeneration coupling with SMSCs.
最近，人们认为伤口附近的组织是一种很有前途的治疗细胞来源，可以用于治疗和修复目的。具体来说，已经在滑膜组织（一种与关节软骨相邻的组织）中鉴定出治疗性干细胞。本研究旨在探索用包封在光聚合水凝胶中的兔滑膜间充质干细胞（SMSCs）进行治疗性软骨生成。应用不可降解的聚乙二醇二丙烯酸酯（PEGDA）基水凝胶和可生物降解的磷酸酯-聚乙二醇（PhosPEG）基水凝胶作为 3-D 支架，在体外介导 SMSC 软骨生成。通过荧光死活检测和 WST-1 检测评估 SMSC 在两种水凝胶中的活力。通过实时 RT-PCR、生化分析和免疫组织化学分析分别评估 SMSC 软骨生成特异性的基因和蛋白水平。结果表明，SMSCs 包封在水凝胶中时仍具有很高的活力。经转化生长因子（TGF）-β1 或 TGF-β3 处理，两种凝胶中均成功实现了 SMSC 的正向软骨生成，PEGDA 系统的效果最佳。可以得出结论，PEGDA 和 PhosPEG 水凝胶都是 SMSC 软骨生成的合适细胞输送载体。特别是作为一种可生物降解材料，PhosPEG 水凝胶在未来与 SMSCs 结合用于关节软骨再生的应用中具有巨大的潜力。
