腹主动脉瘤破裂与动脉瘤壁内炎症的上调无关。

Abdominal aortic aneurysm rupture is not associated with an up-regulation of inflammation within the aneurysm wall.

机构信息

Department of Vascular Surgery, University Hospital Nottingham, UK.

出版信息

Eur J Vasc Endovasc Surg. 2010 Aug;40(2):191-5. doi: 10.1016/j.ejvs.2010.04.014. Epub 2010 Jun 2.

DOI:10.1016/j.ejvs.2010.04.014

PMID:20537568

Abstract

BACKGROUND

The nature of the inflammatory change within ruptured AAA has not been extensively reported. The aim of this study was to compare the inflammatory response in non-ruptured and ruptured aneurysms with emphasis on the site of rupture.

METHODS

Non-rupture site biopsies were taken from the anterior aneurysm sac of non-ruptured (n=31) and ruptured AAA (n=20). In 12 ruptured AAA, a further biopsy was taken from the rupture site. Enzyme-linked immunosorbent assay was used to quantify IL-6, IL-1beta and TNF-alpha. Quantitative immunohistochemistry was undertaken for generic lymphocytes, T-cells, and B-cells.

RESULTS

Comparing biopsies in non-ruptured AAA versus a non-rupture site biopsy from ruptured AAA; there was no significant difference in IL-6, IL-1beta, TNF-alpha, generic lymphocytes, T-cell or B-cell content. Comparing ruptured AAA--non-rupture site with rupture site; IL-6 and TNF-alpha were unchanged. By contrast IL-1beta and lymphocytes were lower at the rupture site compared to the non-rupture site (IL-1beta 1.39 ng/mg [0.97-2.29] vs. 1.92 ng/mg [1.46-2.57], p=0.027; generic lymphocytes 2.89% [0.51-5.51] vs. 4.73% [2.27-12.40], p=0.018; T-cells 0.28% [0.04-1.18] vs. 0.82% [0.40-1.36], p=0.027; B-cells 0.16% [0.04-1.14] vs. 1.30% [0.32-5.40], p=0.021).

CONCLUSIONS

These findings suggest the biological events leading to AAA rupture may not be dependent on an up-regulation in the inflammatory process.

摘要

背景

破裂的腹主动脉瘤内炎症变化的性质尚未得到广泛报道。本研究的目的是比较非破裂性和破裂性动脉瘤的炎症反应，并强调破裂部位。

方法

从非破裂性（n=31）和破裂性腹主动脉瘤（n=20）的前动脉瘤囊中采集非破裂部位活检。在 12 例破裂性腹主动脉瘤中，从破裂部位进一步采集活检。采用酶联免疫吸附试验测定白细胞介素 6、白细胞介素 1β和肿瘤坏死因子-α。进行定量免疫组织化学分析以确定通用淋巴细胞、T 细胞和 B 细胞。

结果

将非破裂性 AAA 与破裂性 AAA 的非破裂部位活检进行比较；白细胞介素 6、白细胞介素 1β、肿瘤坏死因子-α、通用淋巴细胞、T 细胞或 B 细胞含量无显著差异。与破裂部位相比，破裂性 AAA-非破裂部位的白细胞介素 6 和肿瘤坏死因子-α无变化。相反，与非破裂部位相比，破裂部位的白细胞介素 1β和淋巴细胞水平较低（白细胞介素 1β 1.39ng/mg[0.97-2.29] 比 1.92ng/mg[1.46-2.57]，p=0.027；通用淋巴细胞 2.89%[0.51-5.51] 比 4.73%[2.27-12.40]，p=0.018；T 细胞 0.28%[0.04-1.18] 比 0.82%[0.40-1.36]，p=0.027；B 细胞 0.16%[0.04-1.14] 比 1.30%[0.32-5.40]，p=0.021）。