Newman K M, Jean-Claude J, Li H, Ramey W G, Tilson M D
Columbia University, St Luke's/Roosevelt Hospital Center, Department of Surgery, New York, NY 10019.
Circulation. 1994 Nov;90(5 Pt 2):II224-7.
Abdominal aortic aneurysm (AAA) disease is characterized by an increase in proteolysis and loss of matrix components. The cytokines tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta), products of activated macrophages and T cells, are known to increase the production of matrix-degrading enzymes in some pathological states.
Seven AAA and five control aortic tissue extracts were assayed for TNF-alpha and IL-1 beta with ELISA. TNF-alpha was elevated significantly in AAA extracts compared with controls (86 +/- 34 pg/mg of total protein versus 1 +/- 1 pg/mg of total protein; P < .001). IL-1 beta concentration also was significantly increased in the AAA specimens (48 +/- 14 pg/mg of total protein versus 12 +/- 5 pg/mg of total protein; P < .05). Immunoblotting demonstrated secreted forms of TNF-alpha in the AAA extracts, and possible membrane-bound forms were observed when the tissues were detergent-extracted. Known forms of IL-1 beta also were observed on immunoblots of AAA tissue extracts.
The presence of TNF-alpha and IL-1 beta in AAA tissue underscores the importance of the infiltrating inflammatory cells present in the media and adventitia of aneurysmal aortic wall and further implicates an inflammatory process in the pathogenesis of AAA.
腹主动脉瘤（AAA）疾病的特征是蛋白水解增加和基质成分丧失。细胞因子肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）是活化巨噬细胞和T细胞的产物，已知在某些病理状态下会增加基质降解酶的产生。
采用酶联免疫吸附测定法（ELISA）检测7份AAA和5份对照主动脉组织提取物中的TNF-α和IL-1β。与对照组相比，AAA提取物中的TNF-α显著升高（86±34 pg/mg总蛋白 vs 1±1 pg/mg总蛋白；P<.001）。AAA标本中的IL-1β浓度也显著增加（48±14 pg/mg总蛋白 vs 12±5 pg/mg总蛋白；P<.05）。免疫印迹显示AAA提取物中有分泌形式的TNF-α，当用去污剂提取组织时观察到可能的膜结合形式。在AAA组织提取物的免疫印迹上也观察到已知形式的IL-1β。
AAA组织中存在TNF-α和IL-1β突出了动脉瘤主动脉壁中膜和外膜浸润性炎症细胞的重要性，并进一步提示炎症过程在AAA发病机制中的作用。
