Nipate Sonali S, Tiwari Anand H
Department of Pharmacology, P. E. S.'s Modern College of Pharmacy, Sector No. 21, Yamunanagar, Nigdi, Pune 411044, India.
Department of Pharmacology, P. E. S.'s Modern College of Pharmacy, Sector No. 21, Yamunanagar, Nigdi, Pune 411044, India.
J Ayurveda Integr Med. 2020 Apr-Jun;11(2):124-130. doi: 10.1016/j.jaim.2017.08.008. Epub 2018 Nov 16.
Chronic fatigue syndrome (CFS) holds a mystery for researchers due to its multifactorial nature; hence, its diagnosis is still based on symptoms and aetiology remains obscured. Number of scientific evidences regarding the role of oxidative stress, immune dysfunction in CFS and alleviation of symptoms with the help of nutritional supplements guided us to study effect of ethanolic extract of Spilanthes oleracea (SPE) in CFS.
Present study was designed to evaluate antioxidant, immunomodulatory properties of S. oleracea flower to ameliorate CFS infirmity in mice.
In order to induce fatigue, experimental animals were stressed by chronic water - immersion stress model. Meanwhile, parameters like immobility period and tail withdrawal latency were assessed. On the 21st day, mice blood was collected and they were immediately sacrificed for biochemical estimations.
Biochemical analysis results revealed that CFS elevates lipid peroxidation, nitrite level and diminishes the endogenous antioxidant enzyme like catalase level in stressed animal's brain homogenate. Stressful condition developed muscle fatigue leading in alteration of lactate dehydrogenase level (LDH), Blood urea nitrogen (BUN) and Triglycerides (TG) levels. Concurrent and chronic treatment of SPE for 21 days restored all these behavioural despairs and associated biochemical adaptation in mice in dose-dependent manner.
The outcome of this study indicates ability of SPE in amelioration of CFS by mitigating the oxidative stress and thus provide a powerful combat against CFS which may be due to its antioxidant and immunomodulatory properties.
慢性疲劳综合征（CFS）因其多因素性质而令研究人员感到困惑；因此，其诊断仍基于症状，病因仍不明确。关于氧化应激、免疫功能障碍在慢性疲劳综合征中的作用以及营养补充剂对症状缓解作用的大量科学证据，引导我们研究了金纽扣乙醇提取物（SPE）对慢性疲劳综合征的影响。
本研究旨在评估金纽扣花的抗氧化、免疫调节特性，以改善小鼠的慢性疲劳综合征虚弱状态。
为诱导疲劳，通过慢性水浸应激模型对实验动物进行应激处理。同时，评估不动时间和尾撤离潜伏期等参数。在第21天，采集小鼠血液并立即处死以进行生化测定。
生化分析结果显示，慢性疲劳综合征会增加应激动物脑匀浆中的脂质过氧化、亚硝酸盐水平，并降低内源性抗氧化酶如过氧化氢酶水平。应激状态会导致肌肉疲劳，从而改变乳酸脱氢酶水平（LDH）、血尿素氮（BUN）和甘油三酯（TG）水平。连续21天同时给予金纽扣乙醇提取物能以剂量依赖方式恢复小鼠所有这些行为异常及相关的生化适应性变化。
本研究结果表明，金纽扣乙醇提取物能够通过减轻氧化应激来改善慢性疲劳综合征，从而为对抗慢性疲劳综合征提供有力手段，这可能归因于其抗氧化和免疫调节特性。
