Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin, PR China.
Bioresour Technol. 2010 Oct;101(19):7462-71. doi: 10.1016/j.biortech.2010.04.100. Epub 2010 May 26.
The optimal conditions for extraction of astragalosides III and IV (AGs III and IV) in Radix Astragali by negative pressure cavitation-accelerated enzyme pretreatment were studied on the basis of a Box-Behnken design and response surface methodology. Experimental results showed that negative pressure, amount of enzyme and incubation temperature were the main factors governing the enzyme pretreatment of Radix Astragali. The optimum parameters were obtained as follows: negative pressure -0.08 Mpa, amount of enzyme 1.48% (w/w of materials) and incubation temperature 45 degrees C. Under the optimal conditions, the maximal extraction yields of AGs III and IV were 0.103 and 0.325 mg/g, which were 41.67% and 65.31% increased as compared to those without enzyme pretreatment, respectively. The effect of negative pressure cavitation and enzyme pretreatment on the structural changes of plant cells was observed by scanning electron microscopy. In conclusion, negative pressure cavitation-accelerated enzyme pretreatment was proved to be environment-friendly and economical, and could be used in secondary metabolites production.
基于 Box-Behnken 设计和响应面法，研究了减压空化加速酶预处理提取黄芪中黄芪甲苷 III 和 IV（AGs III 和 IV）的最佳条件。实验结果表明，负压、酶用量和孵育温度是影响黄芪酶预处理的主要因素。最优参数如下：负压-0.08 MPa，酶用量 1.48%（w/w 材料），孵育温度 45°C。在最佳条件下，AGs III 和 IV 的最大提取率分别为 0.103 和 0.325mg/g，分别比未经酶预处理提高了 41.67%和 65.31%。通过扫描电子显微镜观察了减压空化和酶预处理对植物细胞结构变化的影响。总之，减压空化加速酶预处理具有环保和经济的特点，可用于次生代谢产物的生产。
