Tian Hao, Li Wan-Yi, Xiao Dan, Li Zhi-Min, Wang Jian-Wen
College of Pharmaceutical Sciences, Soochow University, Suzhou 215123, China.
Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650205, China.
Molecules. 2015 Jun 15;20(6):11076-89. doi: 10.3390/molecules200611076.
The negative-pressure cavitation extraction (NPCE) technique was applied firstly to extract secoisolariciresinol diglucoside (SDG) from flaxseed cakes. The significant extraction parameters were screened by fractional factorial design (FFD). The optimal parameters were determined using the central composite design (CCD) with the two variables, NaOH amount and the liquid/solid ratio. The conditions of the extraction were optimized by using response surface methodology (RSM). Under the optimal conditions, the extraction yield and the extraction purity of SDG was 16.25 mg/g and 3.86%, respectively. The efficiency of NPCE was compared with that of conventional extraction methods. Our results demonstrated that NPCE was comparable to the well-known ultrasound-assisted extraction in term of extraction yield and purity. This extraction technique has advantages of less time-consuming, low solvent usage and high throughput capability.
首次应用负压空化萃取（NPCE）技术从亚麻籽饼中提取开环异落叶松脂素二葡萄糖苷（SDG）。通过分数析因设计（FFD）筛选重要的萃取参数。使用中心复合设计（CCD），以氢氧化钠用量和液固比这两个变量确定最佳参数。采用响应面法（RSM）优化萃取条件。在最佳条件下，SDG的萃取产率和萃取纯度分别为16.25 mg/g和3.86%。将NPCE的效率与传统萃取方法进行比较。我们的结果表明，在萃取产率和纯度方面，NPCE与著名的超声辅助萃取相当。这种萃取技术具有耗时少、溶剂用量低和高通量能力的优点。
