Department of Preventive and Social Dentistry, Graduate School, Kyung Hee University, Hoegi-dong, Dongdaemoon-gu, Seoul 130-701, South Korea.
J Chromatogr A. 2012 Apr 6;1232:212-7. doi: 10.1016/j.chroma.2011.12.035. Epub 2011 Dec 16.
Astragalin and astragalosides were measured in Radix Astragali using reversed-phase chromatography coupled with pulsed amperometric detection. Because the target compounds showed poor stability in aqueous solutions, they were extracted in 100% methanol under reflux. All compounds were detected with high sensitivity under highly alkaline conditions using sodium hydroxide as a post-column eluent. The limits of detection and quantification of target compounds were 0.02-0.36 μg/mL and 0.06-1.09 μg/mL, respectively, and the linear regression coefficients were 0.9982-1.0000. The intra- and inter-day precisions were <0.92% in retention time and <4.78% in calculated contents. Average recoveries were >91.33%. Astragalin and astragaloside contents between Radix Astragali at different ages and in different parts were successfully determined without sample purification or concentration.
采用反相色谱-脉冲安培检测法测定黄芪中的毛蕊异黄酮葡萄糖苷和黄芪甲苷。由于目标化合物在水溶液中稳定性差，在回流下用 100%甲醇提取。所有化合物在高碱性条件下均采用氢氧化钠作为柱后洗脱液进行检测，具有很高的灵敏度。目标化合物的检测限和定量限分别为 0.02-0.36μg/mL 和 0.06-1.09μg/mL，线性回归系数为 0.9982-1.0000。日内和日间精密度分别在保留时间<0.92%和计算含量<4.78%。平均回收率>91.33%。无需样品净化或浓缩，即可成功测定不同年龄和不同部位黄芪中的毛蕊异黄酮葡萄糖苷和黄芪甲苷含量。
