García-de la Torre I, Ramírez-Casillas A, Hernández-Vazquez L
Department of Immunology and Rheumatology, Hospital General de Occidente de la S.S., Zapopan, Jalisco, Mexico.
Arthritis Rheum. 1991 Jun;34(6):744-50. doi: 10.1002/art.1780340616.
In 1986, we diagnosed and treated 4 patients, all members of 1 rural family, who presented simultaneously with a polymyositis-like syndrome, anticytoplasmic antibodies (on HEp-2 cells), and precipitating antibodies of anti-Jo-1 and anti-PM-Scl specificities. Serum samples from these patients reacted by immunodiffusion against serum from a rodent that had been caught in the patients' house. The precipitin line showed complete immunologic identity with anti-Jo-1. To study the specificity of this serum-serum reaction, 2 mice (Mus musculus) and 1 rat (Rattus rattus) from the neighboring areas of the patients' house were caught and killed. Sections of the liver, kidney, spleen, and skeletal muscle were processed for histopathologic examination and for direct and indirect immunofluorescence studies of the patients' sera. The rodents' sera were tested by immunodiffusion against serum samples from 25 patients with systemic lupus erythematosus, 11 with rheumatoid arthritis, 6 with mixed connective tissue disease, and 11 with various other rheumatic diseases, as well as 16 healthy controls. A serum-serum precipitin reaction was noted between the rodents' sera and sera from 2 of the patients with polymyositis-like syndrome. A distinct, but weaker, reaction was found with 2 lupus patients' sera (8%), and with 1 healthy control serum (6%). The rodents' sera were antinuclear antibody positive by indirect immunofluorescence on HEp-2 cells. These results, suggest that some rodents could be carriers of an as-yet-unknown transmissible agent that, in susceptible individuals, induces clinical and serologic manifestations similar to those of polymyositis.
1986年，我们诊断并治疗了4名患者，他们均来自一个农村家庭，同时出现了类似多发性肌炎的综合征、抗细胞质抗体（在HEp-2细胞上）以及具有抗Jo-1和抗PM-Scl特异性的沉淀抗体。这些患者的血清样本通过免疫扩散与在患者家中捕获的一只啮齿动物的血清发生反应。沉淀线显示与抗Jo-1具有完全的免疫同一性。为了研究这种血清-血清反应的特异性，从患者家附近地区捕获并杀死了2只小鼠（小家鼠）和1只大鼠（褐家鼠）。对肝脏、肾脏、脾脏和骨骼肌切片进行组织病理学检查以及患者血清的直接和间接免疫荧光研究。用免疫扩散法检测这些啮齿动物的血清与25例系统性红斑狼疮患者、11例类风湿关节炎患者、6例混合性结缔组织病患者、11例其他各种风湿性疾病患者以及16名健康对照者的血清样本。在啮齿动物的血清与2例类似多发性肌炎综合征患者的血清之间发现了血清-血清沉淀反应。在2例狼疮患者的血清（8%）和1例健康对照者的血清（6%）中发现了明显但较弱的反应。通过在HEp-2细胞上的间接免疫荧光检测，这些啮齿动物的血清抗核抗体呈阳性。这些结果表明，一些啮齿动物可能是一种尚未知晓的可传播病原体的携带者，在易感个体中，该病原体可诱发与多发性肌炎相似的临床和血清学表现。
