Reichlin M, Arnett F C
Arthritis Rheum. 1984 Oct;27(10):1150-6. doi: 10.1002/art.1780271011.
Serologic studies on 114 patients with polymyositis and dermatomyositis revealed that 89% had either a precipitating antibody to antigens in calf thymus extract or a positive immunofluorescent reaction on HEp-2 cells, a human tissue culture line. Previously, the greatest proportion of polymyositis sera demonstrating positive serologic results (i.e., the proportion of patients' sera forming precipitates with calf thymus extract) was reported to be 60%. Use of the HEp-2 cell as immunofluorescent substrate enabled the detection of antibody in 89 (78%) of the sera, providing the additional probe which demonstrated specific antibody. Nuclear, cytoplasmic, and nucleolar staining are the most common patterns of immunofluorescence. The immunofluorescent patterns and individual precipitin reactions are related to each other and to the clinical syndromes in which they appear.
对114例多发性肌炎和皮肌炎患者进行的血清学研究显示，89%的患者对小牛胸腺提取物中的抗原产生沉淀抗体，或对人组织培养细胞系HEp-2细胞产生阳性免疫荧光反应。此前，血清学检测结果呈阳性（即患者血清与小牛胸腺提取物形成沉淀）的多发性肌炎血清的最大比例据报道为60%。使用HEp-2细胞作为免疫荧光底物，在89份（78%）血清中检测到抗体，提供了可证明特异性抗体的额外检测方法。核染色、胞质染色和核仁染色是最常见的免疫荧光模式。免疫荧光模式和个体沉淀反应相互关联，也与它们出现的临床综合征相关。
