Wolfe J F, Adelstein E, Sharp G C
J Clin Invest. 1977 Jan;59(1):176-8. doi: 10.1172/JCI108616.
In the course of studying antinuclear antibodies in the rheumatic diseases, a new precipitin reaction (provisionally referred to as PM-1) was observed between calf thymus nuclear extract and polymyositis sera. Objectives of this study were to further define the immunologic nature of this reaction and to determine its specificity for polymyositis. Immunodiffusion studies using calf thymus nuclear extract revealed the PM-1 precipitin line in 17 of 28 patients with polymyositis. This reaction was not produced by sera of 460 patients with other diseases. Enzyme and heat treatments of the nuclear extract showed that PM-1 was distinct from native DNA, ribonucleoprotein, and Sm antigens. Fractionation of PM-1-positive serum by 30% ammonium sulphate and Sephadex G-200 chromatography revealed that the factor producing the PM-1 precipitin reaction was in a serum fraction which showed only IgG by immunoelectrphoresis against anti-whole human serum. Because of the apparent strong specificity, the PM-1 system may represent a marker antibody for polymyositis.
在研究风湿性疾病中的抗核抗体过程中，在小牛胸腺核提取物与多发性肌炎血清之间观察到一种新的沉淀反应（暂称为PM-1）。本研究的目的是进一步确定该反应的免疫学性质，并确定其对多发性肌炎的特异性。使用小牛胸腺核提取物进行的免疫扩散研究显示，28例多发性肌炎患者中有17例出现PM-1沉淀线。460例其他疾病患者的血清未产生此反应。对核提取物进行酶处理和热处理表明，PM-1与天然DNA、核糖核蛋白和Sm抗原不同。用30%硫酸铵和Sephadex G-200色谱法对PM-1阳性血清进行分级分离，结果显示产生PM-1沉淀反应的因子存在于血清组分中，该组分通过针对抗全人血清的免疫电泳仅显示IgG。由于明显的强特异性，PM-1系统可能代表多发性肌炎的一种标记抗体。
