Mendelson Y, Lin B C, Peura R A, Clermont A C
Appl Opt. 1988 Dec 15;27(24):5077-81. doi: 10.1364/AO.27.005077.
Conventional IR transmission methods are not suitable to measure accurately the concentration of biological molecules in aqueous solutions since the dominant water component has a high intrinsic absorption band over a wide range of the IR spectrum. This limitation can be largely overcome by the use of a laser as a light source. This paper describes the design of an experimental IR spectrometer based on a CO(2) laser and an ATR technique. To demonstrate the applicability of this laser spectrometer in medical diagnosis, we compared quantitative measurements of D-glucose solutions obtained from the IR spectrometer with measurements made by a standard laboratory glucose analyzer.
传统的红外传输方法不适用于精确测量水溶液中生物分子的浓度，因为占主导的水成分在红外光谱的很宽范围内具有很高的固有吸收带。使用激光作为光源可在很大程度上克服这一限制。本文描述了一种基于二氧化碳激光和衰减全反射（ATR）技术的实验性红外光谱仪的设计。为了证明这种激光光谱仪在医学诊断中的适用性，我们将红外光谱仪对D -葡萄糖溶液的定量测量结果与标准实验室葡萄糖分析仪的测量结果进行了比较。
