Sharlaimova N S, Pinaev G P, Petukhova O A
Tsitologiia. 2010;52(4):317-25.
Proposed sources of coelomocytes in Asteroidea after traumatic injures are coelomic epithelium, axial organ or Tidemann's bodies. To study the involvement of cell division in the process, proliferation of cells from different tissues of starfish Asterias rubens L. has been studied after bromdeoxyuridine incorporation in vivo. To study the differentiation of coelomocytes in vitro a method for isolation and cultivation of different tissue cells has been worked out and cell behaviour and proliferation in culture has been analyzed. The reliable BrdU incorporation has been found in coelomic epithelium cells in vivo. Coelomocytes and coelomic epithelium cells behaviour in culture dependent on the post-trauma period after which the cells were loaded into the culture whereas no difference was revealed for axial organ and Tidemann's bodies cells. Two-month cultivation of coelomic epithelium cells resulted in formation of colony-like accumulations of the cells with high nuclear-cytoplasm ratio which of colony-like accumulation of the cells with high nuclear-cytoplasm ratio which incorporated BrdU. Thus, coelomic epithelium cells seem to be more promising object for the study of A. rubens cell differentiation in vitro.
海星纲动物在受到创伤后，拟议的体腔细胞来源是体腔上皮、轴器官或蒂德曼氏体。为了研究细胞分裂在这一过程中的参与情况，在体内掺入溴脱氧尿苷后，对红海星不同组织的细胞增殖进行了研究。为了在体外研究体腔细胞的分化，已经制定了一种分离和培养不同组织细胞的方法，并分析了培养中的细胞行为和增殖情况。在体内体腔上皮细胞中发现了可靠的溴脱氧尿苷掺入。培养中的体腔细胞和体腔上皮细胞行为取决于将细胞接种到培养物中的创伤后时期，而轴器官和蒂德曼氏体细胞则未显示出差异。体腔上皮细胞培养两个月后，形成了细胞核质比高的细胞集落样聚集物，这些细胞集落样聚集物掺入了溴脱氧尿苷。因此，体腔上皮细胞似乎是体外研究红海星细胞分化更有前景的对象。
