Collection of Vertebrate Cell Cultures, Institute of Cytology, Russian Academy of Sciences, Tikhoretsky Ave. 4, St. Petersburg, Russian Federation, 194064.
Cell Technologies Centre, Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russian Federation.
Cell Tissue Res. 2021 Mar;383(3):1043-1060. doi: 10.1007/s00441-020-03337-z. Epub 2020 Nov 25.
The origin of cells involved in regeneration in echinoderms remains an open question. Replenishment of circulatory coelomocytes-cells of the coelomic cavity in starfish-is an example of physiological regeneration. The coelomic epithelium is considered to be the main source of coelomocytes, but many details of this process remain unclear. This study examined the role of coelomocytes outside circulation, named marginal coelomocytes and small undifferentiated cells of the coelomic epithelium in coelomocyte replenishment in Asterias rubens. A qualitative and quantitative comparison of circulatory and marginal coelomocytes, as well as changes of circulatory coelomocyte concentrations in response to injury at different physiological statuses, was analysed. The presence of cells morphologically similar to coelomocytes in the context of coelomic epithelium was evaluated by electron microscopy. The irregular distribution of small cells on the surface and within the coelomic epithelium was demonstrated and the origin of small undifferentiated cells and large agranulocytes from the coelomic epithelium was suggested. Two events have been proposed to mediate the replenishment of coelomocytes in the coelom: migration of mature coelomocytes of the marginal cell pool and migration of small undifferentiated cells of the coelomic epithelium. The proteomic analysis of circulatory coelomocytes, coelomic epithelial cells and a subpopulation of coelomic epithelial cells, enriched in small undifferentiated cells, revealed proteins that were common and specific for each cell pool. Among these molecules were regulatory proteins, potential participants of regenerative processes.
棘皮动物中参与再生的细胞的起源仍然是一个悬而未决的问题。循环体腔细胞——海星体腔中的细胞——的补充是生理再生的一个例子。体腔上皮被认为是体腔细胞的主要来源，但这个过程的许多细节仍不清楚。本研究检查了循环体外的体腔细胞，即边缘体腔细胞和体腔上皮的小未分化细胞，在 Asterias rubens 中体腔细胞补充中的作用。分析了循环和边缘体腔细胞的定性和定量比较，以及循环体腔细胞浓度在不同生理状态下对损伤的反应变化。通过电子显微镜评估了体腔上皮中形态上类似于体腔细胞的细胞的存在。证明了小细胞在体腔上皮表面和内部的不规则分布，并提出了小未分化细胞和大颗粒细胞来源于体腔上皮的观点。提出了两种事件来介导体腔细胞在体腔中的补充：边缘细胞池中成熟体腔细胞的迁移和体腔上皮中未分化小细胞的迁移。循环体腔细胞、体腔上皮细胞和富含小未分化细胞的体腔上皮细胞亚群的蛋白质组学分析揭示了每个细胞群共有的和特定的蛋白质。这些分子中有调节蛋白，它们是再生过程的潜在参与者。
