Kameda E, Langone M A P, Coelho M A Z
Escola de Química, Universidade Federal do Rio de Janeiro, Centro de Tecnologia, Bloco E, Lab 113, Cidade Universitária, CEP 21949-900, Rio de Janeiro-RJ, Brasil.
Environ Technol. 2006 Nov;27(11):1209-15. doi: 10.1080/09593332708618736.
Phenols are toxic pollutants found in industrial wastes imposing several risks to human health. Tyrosinase (EC 1.14.18.1) is an oxygenase oxyreductase found in several life forms, like the mushroom Agaricus bisporus. This enzyme is readily available from this fungal tissue leading to high activity extracts without extensive purification, thus suggesting its potential as a biocatalyst for applications involving biomodification of phenols or bioremediation of phenol-polluted waters. The purpose of this work was to employ a crude extract from the Agaricus bisporus mushroom and its biomass for the removal of phenol from polluted water. Experiments were carried out without pH control. The initial phenol concentration in all solutions was 100 mg l(-1). Four enzymatic concentrations (50, 100, 200 and 400 U ml(-1)) were tested. Reactions, with 200 U ml(-1) and 400 U ml(-1) enzymatic activity, led to 90% of phenol removal. Chitosan was used as a coagulant, but no significant improvement was observed. The in natura fungi was also able to remove 90% of phenol, demostrating its viability as a biocatalyst in bioremediation process.
酚类是工业废物中发现的有毒污染物，对人类健康构成多种风险。酪氨酸酶（EC 1.14.18.1）是一种加氧氧化还原酶，存在于多种生命形式中，如双孢蘑菇。这种酶很容易从这种真菌组织中获得，无需大量纯化就能得到高活性提取物，因此表明其作为生物催化剂在涉及酚类生物改性或酚类污染水生物修复的应用中的潜力。这项工作的目的是利用双孢蘑菇的粗提物及其生物质从污染水中去除苯酚。实验在没有pH控制的情况下进行。所有溶液中的初始苯酚浓度为100 mg l(-1)。测试了四种酶浓度（50、100、200和400 U ml(-1)）。酶活性为200 U ml(-1)和400 U ml(-1)的反应导致90%的苯酚被去除。壳聚糖用作凝聚剂，但未观察到显著改善。天然真菌也能够去除90%的苯酚，证明了其作为生物修复过程中生物催化剂的可行性。
