Department of Clinical Pharmacy, Shiraz Pharmaceutical Research Center, Faculty of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Islamic Republic of Iran.
Ren Fail. 2010 Jul;32(6):733-9. doi: 10.3109/0886022X.2010.486488.
To investigate the effect of silymarin and milk thistle extract on the progression of diabetic nephropathy (DN) in rats.
Diabetes was induced with a single intraperitoneal (IP) injection of streptozotocin (STZ) (60 mg/kg). Silymarin (100 mg/kg/d) or the extract (1.2 g/kg/d) was gavaged for 4 weeks. Blood glucose (BS), serum urea (S(u)), serum creatinine (S(cr)), and 24-h urine protein (Up) were measured and glomerular filtration rate (GFR) was calculated. Concentration of thiobarbituric acid reactive species (TBARS) and activities of glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase (CAT) were evaluated in the renal tissue.
Data were expressed as mean +/- SEM. Silymarin or the extract had no significant effect on BS, S(cr), and GFR. Both milk thistle extract and silymarin, respectively, decreased S(u) (mg/dL) (87.1 +/- 7.78, p < 0.001; 84.5 +/- 7.15, p < 0.001), Up (mg) (5.22 +/- 1.56, p = 0.014; 5.67 +/- 0.86, p = 0.034), and tissue TBARS (nmol/mg protein) (0.67 +/- 0.04, p < 0.001; 0.63 +/- 0.07, p < 0.001) in diabetic rats, compared to diabetic control (DC) (S(u): 131.0 +/- 4.55, Up: 8.3 +/- 0.84, TBARS: 0.94 +/- 0.06). Both the extract and silymarin could increase the activity of CAT (IU/mg protein) (25.5 +/- 4.0, p = 0.005; 20 +/- 1.8, p = 0.16) and GPx (IU/mg protein) (0.86 +/- 0.05, p = 0.005; 0.74 +/- 0.04, p = 0.10), respectively, in diabetic rats compared to DC (CAT = 14.4 +/- 2.0, GPx = 0.57 +/- 0.02).
Milk thistle extract, to a lesser extent silymarin, can attenuate DN in rats possibly by increasing kidney CAT and GPx activity and decreasing lipid peroxidation in renal tissue.
研究水飞蓟素和奶蓟草提取物对糖尿病肾病（DN）大鼠进展的影响。
通过单次腹腔（IP）注射链脲佐菌素（STZ）（60mg/kg）诱导糖尿病。水飞蓟素（100mg/kg/d）或提取物（1.2g/kg/d）灌胃 4 周。测量血糖（BS）、血清尿素（S（u））、血清肌酐（S（cr））和 24 小时尿蛋白（Up），并计算肾小球滤过率（GFR）。评估肾组织中丙二醛（TBARS）的浓度和谷胱甘肽过氧化物酶（GPx）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的活性。
数据表示为平均值 +/- SEM。水飞蓟素或提取物对 BS、S（cr）和 GFR 没有显著影响。奶蓟草提取物和水飞蓟素分别降低了 S（u）（mg/dL）（87.1 +/- 7.78，p < 0.001；84.5 +/- 7.15，p < 0.001）、Up（mg）（5.22 +/- 1.56，p = 0.014；5.67 +/- 0.86，p = 0.034）和组织 TBARS（nmol/mg 蛋白）（0.67 +/- 0.04，p < 0.001；0.63 +/- 0.07，p < 0.001）在糖尿病大鼠中，与糖尿病对照组（DC）相比（S（u）：131.0 +/- 4.55，Up：8.3 +/- 0.84，TBARS：0.94 +/- 0.06）。与 DC 相比，提取物和水飞蓟素均可分别增加 CAT（IU/mg 蛋白）（25.5 +/- 4.0，p = 0.005；20 +/- 1.8，p = 0.16）和 GPx（IU/mg 蛋白）（0.86 +/- 0.05，p = 0.005；0.74 +/- 0.04，p = 0.10）的活性，（CAT = 14.4 +/- 2.0，GPx = 0.57 +/- 0.02）。
奶蓟草提取物在一定程度上水飞蓟素可减轻大鼠的 DN，可能是通过增加肾脏 CAT 和 GPx 活性和减少肾组织脂质过氧化。
