Chlopcíková Sárka, Psotová Jitka, Miketová Petra, Simánek Vilím
Institute of Medical Chemistry and Biochemistry, Faculty of Medicine, Hnevotínská 3, 775 15 Olomouc, Czech Republic.
Phytother Res. 2004 Feb;18(2):107-10. doi: 10.1002/ptr.1415.
Silymarin, an extract of fl avonolignans from the dried fruits of milk thistle (Silybum marianum L. Gaertneri) and its constituents silibinin, dehydrosilibinin, silychristin and silydianin were tested for protective effects on rat cardiomyocytes exposed to doxorubicin. Silymarin and individual fl avonolignans did not exert cytotoxicity in the range 25-100 micro m (incubation 9 h). Dehydrosilibinin was tested only at 25 micro m concentration due to its low solubility. All substances increased the cell ATP level. Silymarin and fl avonolignans displayed a dose-dependent cytoprotection against doxorubicin (100 micro m, incubation 8 h). The protective effects of silymarin, silibinin, dehydrosilibinin and silychristin were comparable to that of dexrasoxane, while silydianin exerted the best protective effect. The ability of silymarin complex and its components to protect cardiomyocytes against doxorubicin-induced oxidative stress is due mainly to their cell membrane stabilization effect, radical scavenging and iron chelating potency.
水飞蓟素是从水飞蓟（Silybum marianum L. Gaertneri）干燥果实中提取的黄酮木脂素，对其成分水飞蓟宾、脱氢水飞蓟宾、水飞蓟素和水飞蓟宁进行了测试，以观察它们对暴露于阿霉素的大鼠心肌细胞的保护作用。水飞蓟素和单个黄酮木脂素在25-100微摩尔范围内（孵育9小时）未表现出细胞毒性。由于脱氢水飞蓟宾溶解度低，仅在25微摩尔浓度下进行了测试。所有物质均提高了细胞ATP水平。水飞蓟素和黄酮木脂素对阿霉素（100微摩尔，孵育8小时）表现出剂量依赖性的细胞保护作用。水飞蓟素、水飞蓟宾、脱氢水飞蓟宾和水飞蓟素的保护作用与右丙亚胺相当，而水飞蓟宁的保护作用最佳。水飞蓟素复合物及其成分保护心肌细胞免受阿霉素诱导的氧化应激的能力主要归因于它们的细胞膜稳定作用、自由基清除和铁螯合能力。
