McGill Centre for Research in Neuroscience, Department of Neurology and Neurosurgery, McGill University Health Centre, 1650 Cedar Avenue, Montreal, Quebec H3G 1A4, Canada.
Mol Brain. 2010 Jun 11;3:19. doi: 10.1186/1756-6606-3-19.
The establishment of tissue architecture in the nervous system requires the proper migration and positioning of newly born neurons during embryonic development. Defects in nuclear translocation, a key process in neuronal positioning, are associated with brain diseases such as lissencephaly in humans. Accumulated evidence suggests that the molecular mechanisms controlling neuronal movement are conserved throughout evolution. While the initial events of neuronal migration have been extensively studied, less is known about the molecular details underlying the establishment of neuronal architecture after initial migration.
In a search for novel players in the control of photoreceptor (R cell) positioning in the developing fly visual system, we found that misexpression of the RabGAP RN-Tre disrupted the apical localization of R-cell nuclei. RN-Tre interacts with Rab5 and Rab11 in the fly eye. Genetic analysis shows that Rab5, Shi and Rab11 are required for maintaining apical localization of R-cell nuclei.
We propose that Rab5, Shi and Rab11 function together in a vesicular transport pathway for regulating R-cell positioning in the developing eye.
神经系统组织架构的建立需要在胚胎发育过程中使新生神经元正确迁移和定位。核易位（神经元定位的关键过程）缺陷与人类无脑回畸形等脑部疾病有关。越来越多的证据表明，控制神经元运动的分子机制在整个进化过程中是保守的。虽然神经元迁移的初始事件已经得到了广泛的研究，但对于初始迁移后建立神经元结构的分子细节知之甚少。
在寻找控制发育中果蝇视觉系统中光感受器（R 细胞）定位的新因子的过程中，我们发现 RabGAP RN-Tre 的过表达破坏了 R 细胞核的顶端定位。RN-Tre 在果蝇眼中与 Rab5 和 Rab11 相互作用。遗传分析表明 Rab5、Shi 和 Rab11 对于维持 R 细胞核的顶端定位是必需的。
我们提出 Rab5、Shi 和 Rab11 一起在囊泡运输途径中发挥作用，以调节发育中眼睛中 R 细胞的定位。
