Post-Graduation Program in Physical Education, Catholic University of Brasilia, Brasilia, 71966-700, DF, Brazil.
Center for Proteomic and Biochemical Analysis, Post-Graduation in Genomic and Biotechnology Sciences, Catholic University of Brasilia, Brasília, 71966-700, DF, Brazil.
Nutrients. 2019 Jan 11;11(1):149. doi: 10.3390/nu11010149.
Several studies have indicated that diet and exercise may modulate the gut microbiota in obese subjects. Both interventions were shown to alter the microbiota orthogonally. However, this relationship has not been fully explored. This study analyzed the effects of low-to-moderate aerobic training on the fecal microbiota of mice subjected to a high-fat diet (HFD). Here, 40 male mice (C57Bl/6) were divided into two groups with standard diet (SD; 12.4% lipid) and HFD (60.3% lipid) for four months. These groups were divided into four, named SD control, HF control, SD trained and HF trained. All animals were submitted to an incremental test to estimate low-to-moderate maximum speed Training consisted of 30 min·day, 5 days/week, for 8 weeks. The HFD increased the body weight ( < 0.0001) and adiposity index ( < 0.05). HFD also negatively influenced performance in exercise training. Moreover, the diversity of gut microbiota was reduced by the HFD in all groups. A low-to-moderate exercise was ineffective in modulating the gut microbiota composition in mice subjected to HFD. These findings suggest that two months of low-to-moderate exercise does not achieve a preponderant modulatory effect on shaping microbiota when submitted to the high-fat diet.
已有多项研究表明，饮食和运动可能调节肥胖人群的肠道微生物群。这两种干预措施都被证明可以正交地改变微生物群。然而，这种关系尚未得到充分探索。本研究分析了低等至中等强度有氧运动对高脂肪饮食（HFD）小鼠粪便微生物群的影响。在这里，40 只雄性小鼠（C57Bl/6）分为两组，分别给予标准饮食（SD；12.4%脂质）和 HFD（60.3%脂质），持续四个月。这些组分为四组，分别命名为 SD 对照组、HF 对照组、SD 训练组和 HF 训练组。所有动物都进行了增量测试，以估计低等至中等最大速度。训练包括 30 分钟·天，每周 5 天，持续 8 周。HFD 增加了体重（<0.0001）和肥胖指数（<0.05）。HFD 还对运动训练中的表现产生了负面影响。此外，HFD 降低了所有组的肠道微生物多样性。低等至中等强度的运动对 HFD 小鼠肠道微生物群组成的调节作用无效。这些发现表明，两个月的低等至中等强度运动并不能在高脂肪饮食的情况下对塑造微生物群产生优势调节作用。
