Beckmann N, Turkalj I, Seelig J, Keller U
MR Center and Biocenter, University of Basel, Switzerland.
Biochemistry. 1991 Jul 2;30(26):6362-6. doi: 10.1021/bi00240a002.
Proton-decoupled 13C NMR spectra of the human head were obtained during hyperglycemic glucose clamping using intravenous infusions of [1-13C]glucose in normal volunteers. In addition to 13C signals of mobile lipids, a variety of new metabolite resonances could be resolved for the first time in the human brain. At an enrichment level of 20% [1-13C]glucose, the signals of alpha- and beta-glucose at 92.7 and 96.6 ppm, respectively, could be detected in the human brain after only an infusion period of 15 min. The spatial localization of the different regions of interest was confirmed by 13C NMR spectroscopic imaging with a time resolution of 9 min. Increasing the enrichment level to 99% [1-13C]glucose not only improved the time resolution but allowed the detection of metabolic breakdown products of [1-13C]glucose. The time course of 13C label incorporation into the C2, C3, and C4 resonances of glutamate/glutamine and into lactate could be recorded in the human brain. These results suggest the possibility of obtaining time-resolved, spatially selective, and chemically specific information on the human body.
在正常志愿者中，通过静脉输注[1-¹³C]葡萄糖进行高血糖葡萄糖钳夹期间，获得了人体头部的质子去耦¹³C核磁共振谱。除了可移动脂质的¹³C信号外，首次在人脑中分辨出了多种新的代谢物共振峰。在[1-¹³C]葡萄糖富集水平为20%时，仅在输注15分钟后，就能在人脑中检测到分别位于92.7 ppm和96.6 ppm处的α-葡萄糖和β-葡萄糖信号。通过时间分辨率为9分钟的¹³C核磁共振光谱成像，确认了不同感兴趣区域的空间定位。将[1-¹³C]葡萄糖的富集水平提高到99%，不仅提高了时间分辨率，还能检测到[1-¹³C]葡萄糖的代谢分解产物。可以记录¹³C标记掺入人脑中谷氨酸/谷氨酰胺的C2、C3和C4共振峰以及乳酸中的时间进程。这些结果表明，有可能获得关于人体的时间分辨、空间选择性和化学特异性信息。
