Behar K L, Petroff O A, Prichard J W, Alger J R, Shulman R G
Magn Reson Med. 1986 Dec;3(6):911-20. doi: 10.1002/mrm.1910030611.
1H-decoupled 13C NMR spectra (20.2 MHz) of the living rabbit brain were collected with a surface coil following the intravenous infusion of [1-13C]glucose. Within 15 min of infusion, the alpha and beta anomers of glucose were detected and, shortly thereafter, the carbon atoms at positions C4, C3, and C2 of glutamate and(or) glutamine. After reductions of inspired oxygen from 30 to 5%, lactate C3 was detected. The intensity of the lactate resonance rose progressively during hypoxia and later fell during recovery with oxygen. The 13C fractional isotopic enrichment of arterial blood glucose was measured by 1H NMR providing information on the rate and extent of blood glucose labeling.
在静脉注射[1-¹³C]葡萄糖后，用表面线圈采集活兔脑的¹H去耦¹³C NMR谱（20.2 MHz）。在注射后15分钟内，检测到葡萄糖的α和β异头物，此后不久，检测到谷氨酸和（或）谷氨酰胺C4、C3和C2位的碳原子。在将吸入氧气从30%降至5%后，检测到乳酸C3。在缺氧期间，乳酸共振强度逐渐升高，随后在恢复吸氧期间下降。通过¹H NMR测量动脉血葡萄糖的¹³C分数同位素富集，提供有关血糖标记速率和程度的信息。
