van Zijl P C, Chesnick A S, DesPres D, Moonen C T, Ruiz-Cabello J, van Gelderen P
Department of Radiology, Johns Hopkins University Medical School, Baltimore, Maryland 21287.
Magn Reson Med. 1993 Nov;30(5):544-51. doi: 10.1002/mrm.1910300504.
Metabolism of [1-13C]-glucose was studied in situ in cat brain using gradient-enhanced proton-detected heteronuclear spectroscopy. Proton detection of [1-13C]-glucose, [3-13C]-lactate, 4-[13C]-glutamine, 4-[13C]-glutamate and the combined signals 2-[13C]-glutamate/glutamine and 3-[13C]-glutamate/glutamine was achieved, despite the fact that some of the associated proton resonances are close to the water signal. Two-dimensional [1H-13C]-spectra demonstrate the possibility of in situ spectral assignment with 1H sensitivity and 13C resolution. Spectroscopic images of glucose and its metabolic products were also acquired, showing the possibility to study spatial dependence of metabolism.
利用梯度增强质子检测异核光谱法，在猫脑原位研究了[1-¹³C]-葡萄糖的代谢。尽管一些相关的质子共振接近水信号，但仍实现了对[1-¹³C]-葡萄糖、[3-¹³C]-乳酸、4-[¹³C]-谷氨酰胺、4-[¹³C]-谷氨酸以及组合信号2-[¹³C]-谷氨酸/谷氨酰胺和3-[¹³C]-谷氨酸/谷氨酰胺的质子检测。二维[¹H-¹³C]光谱证明了以¹H灵敏度和¹³C分辨率进行原位光谱归属的可能性。还获取了葡萄糖及其代谢产物的光谱图像，显示了研究代谢空间依赖性的可能性。
