Goodarzi Mohammad Taghi, Abdi Mohammad, Tavilani Heidar, Nadi Ebrahim, Rashidi Mojtaba
Research Group of Pulmonary Disease and Tuberculosis, Faculty of Medicine, Hamadan University of Medical Science, Hamadan, Iran.
Iran J Allergy Asthma Immunol. 2010 Mar;9(1):7-12.
Chronic obstructive pulmonary disease (COPD) has been defined by the Global Initiative for Chronic Obstructive Lung Disease (GOLD), as a disease state characterized by airflow limitation which is not fully reversible. COPD consists of emphysema which is the destruction and inflammation of the lung alveoli. Adenosine deaminase (ADA, E.C.3.5.4.4) converts adenosine to inosine. There are two isoenzymes of ADA in serum; ADA1 and ADA2. It has been established that in COPD patients the adenosine levels increase, which can contribute to decrease of ADA activity. In this research we studied the ADA and its isoenzyme activity in COPD patients. This descriptive analytical case-control study was performed on thirty patients who were hospitalized in the pulmonary wards with an acute exacerbation of COPD. ADA activity was determined in 30 COPD patients, 30 nonsmokers and 30 smokers controls. All subjects were male. We used colorimetric (Giusti) method for measuring of ADA activity. The data were analyzed using SPSS 13 software and Kruskall-Wallis and two-way ANOVA tests. Total ADA activity in the COPD and smoker control groups was significantly lower than in non smoker group (18.99 -/+ 7, 19.03 -/+ 9.1 and 22.95 -/+ 6.7 U/L, respectively). There was a significant difference for ADA2 between the three groups. Whereas the ADA1 activity in the three groups had no significant difference. Based on the obtained data, decrease of ADA activity may play an important role in the formation of pulmonary injury in COPD patients.
慢性阻塞性肺疾病（COPD）已被慢性阻塞性肺疾病全球倡议组织（GOLD）定义为一种以气流受限为特征且不完全可逆的疾病状态。COPD包括肺气肿，即肺泡的破坏和炎症。腺苷脱氨酶（ADA，E.C.3.5.4.4）将腺苷转化为肌苷。血清中有两种ADA同工酶；ADA1和ADA2。已证实COPD患者体内腺苷水平升高，这可能导致ADA活性降低。在本研究中，我们研究了COPD患者的ADA及其同工酶活性。这项描述性分析病例对照研究对30名因COPD急性加重而入住肺病病房的患者进行。测定了30名COPD患者、30名不吸烟者和30名吸烟者对照组的ADA活性。所有受试者均为男性。我们使用比色法（朱斯蒂法）测量ADA活性。使用SPSS 13软件以及克鲁斯卡尔 - 沃利斯检验和双向方差分析对数据进行分析。COPD组和吸烟者对照组的总ADA活性显著低于不吸烟组（分别为18.99±7、19.03±9.1和22.95±6.7 U/L）。三组之间ADA2存在显著差异。而三组之间ADA1活性无显著差异。基于所得数据，ADA活性降低可能在COPD患者肺损伤的形成中起重要作用。
