肌酸裂解物中一种诱变物质的分离与结构测定

Isolation and structural determination of a mutagenic substance in creatine pyrolysate.

作者信息

Nukaya H, Watanabe H, Ishida H, Tsuji K, Suwa Y, Wakabayashi K, Nagao M, Sugimura T, Kosuge T

机构信息

School of Pharmaceutical Science, University of Shizuoka, Japan.

出版信息

Chem Pharm Bull (Tokyo). 1991 Feb;39(2):533-5. doi: 10.1248/cpb.39.533.

DOI:10.1248/cpb.39.533

PMID:2054883

Abstract

The pyrolysis product of creatine showed potent mutagenic activity to Salmonella typhimurium TA98 with metabolic activation by S9 mix. One of the mutagenic substances in creatine pyrolysate was isolated and named Cre-P-1. Its structure was determined by X-ray crystallography to be 4-amino-1,6-dimethyl-2-methylamino-1H,6H-pyrrolo[3,4-f]benzimidazole- 5,7-dione. Cre-P-1 is the first registered mutagenic heterocyclic amine containing oxygen atoms in the molecule, that has been isolated from pyrolysis products.

摘要

肌酸的热解产物对经S9混合物代谢活化的鼠伤寒沙门氏菌TA98显示出强大的诱变活性。从肌酸热解产物中分离出一种诱变物质并将其命名为Cre-P-1。通过X射线晶体学确定其结构为4-氨基-1,6-二甲基-2-甲氨基-1H,6H-吡咯并[3,4-f]苯并咪唑-5,7-二酮。Cre-P-1是首个从热解产物中分离出来的、分子中含有氧原子的已注册诱变杂环胺。