Hasiów-Jaroszewska Beata, Jackowiak Paulina, Borodynko Natasza, Figlerowicz Marek, Pospieszny Henryk
Department of Virology and Bacteriology, Institute of Plant Protection-National Research Institute, ul. Wegorka 20, Poznań, Poland.
Virus Genes. 2010 Oct;41(2):260-7. doi: 10.1007/s11262-010-0497-0. Epub 2010 Jun 12.
Genetic variability is an essential feature of RNA viruses. It allows them to adapt to the ever-changing environmental conditions. Important biological properties of the viruses, their infectivity, adaptability, and host range, may also depend on the level of quasispecies diversity. Here, we present the analysis of the genetic polymorphism of Pepino mosaic virus (PepMV). The examined populations were isolated from the naturally infected tomato plants (Solanum lycopersicum). In order to determine the complexity of the PepMV populations, the number of different viral variants and their genetic diversity was established. Moreover, phylogenetic trees were created to depict relations between the identified variants. For the first time we have shown that the PepMV exists as a quasispecies. The observed level of genetic variability allows PepMV for a quick and flexible adaptation to different hosts. Our results suggest that the level of PepMV variability possibly influences the course of infection.
基因变异性是RNA病毒的一个基本特征。它使病毒能够适应不断变化的环境条件。病毒的重要生物学特性，如感染性、适应性和宿主范围，也可能取决于准种多样性水平。在此，我们展示了对 Pepino 花叶病毒（PepMV）基因多态性的分析。所检测的群体是从自然感染的番茄植株（Solanum lycopersicum）中分离出来的。为了确定 PepMV 群体的复杂性，我们确定了不同病毒变体的数量及其遗传多样性。此外，还构建了系统发育树来描述所鉴定变体之间的关系。我们首次表明 PepMV 以准种形式存在。观察到的基因变异性水平使 PepMV 能够快速灵活地适应不同宿主。我们的结果表明，PepMV 的变异水平可能影响感染过程。
