Esposito Giuseppe, Pastorino Paolo, Prearo Marino, Magara Gabriele, Cesarani Alberto, Freitas Rosa, Caldaroni Barbara, Meloni Domenico, Pais Antonio, Dondo Alessandro, Antuofermo Elisabetta, Elia Antonia Concetta
The Veterinary Medical Research Institute for Piemonte, Liguria and Valle d'Aosta, 10154 Torino, Italy.
Department of Chemistry, Biology and Biotechnology, University of Perugia, 06123 Perugia, Italy.
Antioxidants (Basel). 2022 Oct 30;11(11):2148. doi: 10.3390/antiox11112148.
Copper (Cu) is a ubiquitous trace element in the aquatic environment, and is usually found at low levels. Copper environmental concentrations can be altered as a result anthropogenic activities. Shellfish are useful bioindicators to ensure adequate environmental monitoring. Thus, the aim of the present study was as follows: (a) determine the LC of copper(I) chloride in grooved carpet shell () collected in the Santa Gilla lagoon (Sardinia, Italy), and (b) analyze the antioxidant biomarkers in digestive gland and gills of same specimens exposed to different concentrations of the above-mentioned metal (0.045, 0.45, and 0.90 mg/L) for 96 h. A withdrawal period of 96 h was considered for the treated clam, carrying out the same biochemical analyses, superoxide dismutase (SOD), catalase (CAT), selenium-dependent glutathione peroxidase (Se-GPx), glutathione S-transferases (GSTs), and total glutathione (GSH+2GSSG) in the two tissues. Different time and dose responses of the antioxidant biomarkers were recorded in the digestive glands and gills. Oxidative stress biomarkers highlighted the ability of Cu to induce oxidative stress in . Clam, following the withdrawal period of 96 h, has not been able to achieve the control levels of all biochemical markers in the digestive gland and gills. can be a suitable model to assess the ecotoxicity of copper in aquatic ecosystems. These findings may advance knowledge on the role and the effects of copper on oxidative stress biomarkers in grooved carpet shell. The metal ecotoxicity response can be useful to perform accurate biomarker-based monitoring programs using this bivalve species.
铜（Cu）是水生环境中普遍存在的微量元素，通常含量较低。由于人为活动，铜的环境浓度可能会发生变化。贝类是确保进行充分环境监测的有用生物指示物。因此，本研究的目的如下：（a）确定在意大利撒丁岛圣吉拉泻湖采集的细纹蚶中氯化亚铜的半数致死浓度，以及（b）分析暴露于上述不同浓度金属（0.045、0.45和0.90 mg/L）96小时的同一标本的消化腺和鳃中的抗氧化生物标志物。对处理后的蛤蜊设定96小时的清除期，对这两个组织进行相同的生化分析，即超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、硒依赖性谷胱甘肽过氧化物酶（Se-GPx）、谷胱甘肽S-转移酶（GSTs）和总谷胱甘肽（GSH+2GSSG）。在消化腺和鳃中记录到抗氧化生物标志物的不同时间和剂量反应。氧化应激生物标志物突出了铜诱导细纹蚶氧化应激的能力。在96小时的清除期后，蛤蜊未能达到消化腺和鳃中所有生化标志物的对照水平。细纹蚶可以作为评估水生生态系统中铜生态毒性的合适模型。这些发现可能会推进关于铜对细纹蚶氧化应激生物标志物的作用和影响的知识。金属生态毒性反应对于使用这种双壳类物种开展基于生物标志物的准确监测计划可能是有用的。
