Department of Molecular Medicine, Byrd Alzheimer's Research Institute, Morsani College of Medicine, University of South Florida, Tampa, FL 33612, USA.
Centro de Investigacao em Materiais Ceramicos e Compositos, Department of Chemistry, 3810-193 Aveiro, Portugal.
Int J Mol Sci. 2024 Jun 7;25(12):6339. doi: 10.3390/ijms25126339.
This review covers the analytical applications of protein partitioning in aqueous two-phase systems (ATPSs). We review the advancements in the analytical application of protein partitioning in ATPSs that have been achieved over the last two decades. Multiple examples of different applications, such as the quality control of recombinant proteins, analysis of protein misfolding, characterization of structural changes as small as a single-point mutation, conformational changes upon binding of different ligands, detection of protein-protein interactions, and analysis of structurally different isoforms of a protein are presented. The new approach to discovering new drugs for a known target (e.g., a receptor) is described when one or more previous drugs are already available with well-characterized biological efficacy profiles.
本文综述了蛋白质在双水相体系（ATPS）中分配的分析应用。我们综述了过去二十年中蛋白质在 ATPS 中分配的分析应用的进展。多个不同应用的例子，如重组蛋白的质量控制、蛋白质错误折叠的分析、单个点突变等结构变化的特征、不同配体结合时构象的变化、蛋白质-蛋白质相互作用的检测以及结构不同的蛋白同工型的分析都有涉及。当一个或多个已知的（例如，受体）药物已经存在且具有特征良好的生物功效谱时，描述了一种用于发现针对已知靶标的新药的新方法。
