牛网格蛋白轻链基因的体细胞图谱：一个新的牛同线群的鉴定。

Somatic cell mapping of bovine clathrin light chain genes: identification of a new bovine syntenic group.

作者信息

Gallagher D S, Threadgill D W, Jackson A P, Parham P, Womack J E

机构信息

Department of Veterinary Pathology, Texas A&M University, College Station 77843.

出版信息

Cytogenet Cell Genet. 1991;56(3-4):154-6. doi: 10.1159/000133074.

DOI:10.1159/000133074

PMID:2055110

Abstract

Two bovine DNA probes (LCa and LCb) complementary to the clathrin light chain genes were hybridized to DNAs from bovine hamster hybrid somatic cell lines retaining different combinations of bovine chromosomes. Concordancy of retention of the clathrin genes was compared to existing syntenic data for the domestic cow. LCb identified a single locus. CLTB, concordant with the genes encoding bovine anti-Müllerian hormone (AMH) and bovine osteonectin from bovine syntenic group U22. LCa recognized two loci. CLTAL1 from a previously unidentified bovine syntenic group. U25, and CLTAL2 which is concordant with GGTB2, a gene marker for bovine syntenic group U18.

摘要

两个与网格蛋白轻链基因互补的牛DNA探针（LCa和LCb）与来自保留不同牛染色体组合的牛-仓鼠杂种体细胞系的DNA进行杂交。将网格蛋白基因保留的一致性与家牛现有的同线数据进行比较。LCb鉴定出一个单一基因座CLTB，它与来自牛同线群U22的编码牛抗苗勒管激素（AMH）和牛骨连接蛋白的基因一致。LCa识别出两个基因座。一个来自先前未鉴定的牛同线群U25的CLTAL1，以及与牛同线群U18的基因标记GGTB2一致的CLTAL2。