El Nahas S M, Oraby H A, de Hondt H A, Medhat A M, Zahran M M, Mahfouz E R, Karim A M
Department of Cell Biology, National Research Center, Dokki, Cairo, Egypt.
Mamm Genome. 1996 Nov;7(11):831-4. doi: 10.1007/s003359900245.
The cosegregation of ten coding loci has been investigated, in a panel of 37 somatic cell hybrids resulting from the fusion of a hamster cell line and river buffalo lymphocytes, by use of Southern hybridization technique. Five syntenic groups, TCRB-PGY3, ASS-ABL, FUCA1P-CRYG, MBP-YES1, and CGN1-ACTA1, previously assigned to cattle as U13, U16, U17, U28, and U29 respectively, were also found to be syntenic in buffalo. Based on the extensive syntenic conservation and banding homology between cattle and river buffalo, comparative mapping predicts the localization of these syntenic groups on river buffalo Chromosomes (Chrs) :BBU7, BBU12, BBU2q, BBU22, and BBU4q respectively as they have been previously localized on cattle Chrs BTA4, BTA11, BTA2, BTA24 & BTA28.
利用Southern杂交技术，在一组由仓鼠细胞系与河水牛淋巴细胞融合产生的37个体细胞杂种中，研究了10个编码基因座的共分离情况。先前分别被指定为牛的U13、U16、U17、U28和U29的五个同线群，即TCRB-PGY3、ASS-ABL、FUCA1P-CRYG、MBP-YES1和CGN1-ACTA1，在水牛中也被发现是同线的。基于牛和河水牛之间广泛的同线保守性和染色体带型同源性，比较定位预测这些同线群分别位于河水牛染色体（Chrs）：BBU7、BBU12、BBU2q、BBU22和BBU4q上，因为它们先前已定位在牛染色体BTA4、BTA11、BTA2、BTA24和BTA28上。
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