Biologisches Institut, Abteilung für Molekularbiologie und Virologie der Pflanzen, Universität Stuttgart, Pfaffenwaldring 57, D-70550 Stuttgart, Germany.
Virology. 2010 Sep 1;404(2):148-57. doi: 10.1016/j.virol.2010.05.003. Epub 2010 Jun 1.
From Brazilian weeds with typical symptoms of a geminivirus infection, the DNAs of two new virus species, two new strains with two variants of already known bipartite begomoviruses were sequenced. Moreover, the first two DNA 1-like satellites (alpha satellites) occurring naturally in the New World were identified. They are related to nanoviral DNA components and show a typical genome organization with one open reading frame coding potentially for a replication-associated protein (Rep), a conserved hairpin structure, and an A-rich region. After coinoculation with their helper begomoviruses (Euphorbia mosaic virus, EuMV or Cleome leaf crumple virus, ClLCrV) the satellite DNAs were transmitted to experimental and natural host plants. Three of the begomovirus isolates (EuMV and ClLCrV) infected Arabidopsis thaliana plants, induced mild symptoms, and one of these (ClLCrV) transreplicated the satellite efficiently. As a result, several novel tools for molecular analyses of this important model plant are provided.
从具有典型双生病毒感染症状的巴西杂草中，测序了两种新病毒种、两种新的已有二分体伴随病毒的变体的 DNA。此外，还鉴定了在新世界中首次自然发生的前两个 DNA1 样卫星（α卫星）。它们与纳米病毒 DNA 成分有关，并显示出典型的基因组组织，其中包含一个开放阅读框，编码潜在的复制相关蛋白（Rep）、保守的发夹结构和富含 A 的区域。与它们的辅助伴生病毒（Euphorbia mosaic virus，EuMV 或 Cleome leaf crumple virus，ClLCrV）共同接种后，卫星 DNA 被传递到实验和自然宿主植物。三种伴生病毒分离物（EuMV 和 ClLCrV）感染拟南芥植物，诱导轻度症状，其中一种（ClLCrV）有效地转复制了卫星。因此，为该重要模式植物的分子分析提供了几个新的工具。
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