Department of Plant Pathology, University of Brasília (UnB), Brasília 70910-900, DF, Brazil.
Embrapa Vegetable Crops (Hortaliças), National Center for Vegetable Crops Research (CNPH),Brasília 70275-970, DF, Brazil.
Viruses. 2024 Jun 1;16(6):899. doi: 10.3390/v16060899.
The diversity of and species in tomatoes was assessed via high-throughput sequencing of 154 symptomatic foliar samples collected from 2002 to 2017 across seven Brazilian biomes. The first pool (BP1) comprised 73 samples from the North (13), Northeast (36), and South (24) regions. Sixteen begomoviruses and one were detected in BP1. Four begomovirus-like contigs were identified as putative novel species (NS). NS#1 was reported in the semi-arid (Northeast) region and NS#2 and NS#4 in mild subtropical climates (South region), whereas NS#3 was detected in the warm and humid (North) region. The second pool (BP2) comprised 81 samples from Southeast (39) and Central-West (42) regions. Fourteen viruses and subviral agents were detected in BP2, including two topileviruses, a putative novel begomovirus (NS#5), and two alphasatellites occurring in continental highland areas. The five putative novel begomoviruses displayed strict endemic distributions. Conversely, tomato mottle leaf curl virus (a monopartite species) displayed the most widespread distribution occurring across the seven sampled biomes. The overall diversity and frequency of mixed infections were higher in susceptible (16 viruses + alphasatellites) in comparison to tolerant (carrying the -1 or -3 introgressions) samples, which displayed 9 viruses. This complex panorama reinforces the notion that the tomato-associated diversity is yet underestimated in Neotropical regions.
通过对 2002 年至 2017 年间从巴西七个生物群落中采集的 154 个有症状叶片样本进行高通量测序，评估了番茄中 和 物种的多样性。第一组（BP1）包括来自北部（13）、东北部（36）和南部（24）的 73 个样本。在 BP1 中检测到 16 种单链 DNA 病毒和一种 。鉴定出四个类单链 DNA 病毒的 contigs 为假定的新种（NS）。NS#1 报道于半干旱（东北部）地区，NS#2 和 NS#4 报道于温和亚热带气候（南部）地区，而 NS#3 则在温暖潮湿（北部）地区被检测到。第二组（BP2）包括来自东南部（39）和中西部（42）的 81 个样本。在 BP2 中检测到 14 种病毒和亚病毒，包括两种双生病毒、一种假定的新单链 DNA 病毒（NS#5）和两种发生在大陆高原地区的阿尔法卫星。这五种假定的新单链 DNA 病毒表现出严格的地方流行分布。相反，番茄斑卷曲病毒（单分体物种）的分布最为广泛，发生在七个采样生物群落。易感（携带 16 种病毒+阿尔法卫星）样本的混合感染多样性和频率高于耐受（携带-1 或-3 渐渗）样本，后者携带 9 种病毒。这种复杂的情况强化了这样一种观点，即番茄相关的多样性在新热带地区仍被低估。
