Department of Molecular and Cellular Biology, Faculty of Sciences, University Abou-Bekr Belkaïd, Tlemcen, Algeria.
Nutr Metab Cardiovasc Dis. 2011 Oct;21(10):792-9. doi: 10.1016/j.numecd.2010.02.007. Epub 2010 Jun 2.
In this study, plasma lipids, lipoproteins and markers of oxidant/antioxidant status were investigated in young (n = 45) and older (n = 40) obese men and compared to those in young (n = 65) and older (n = 55) normal weight controls. The purpose was to determine whether obesity exacerbates or not lipid, lipoprotein abnormalities and oxidative stress in older men. Our findings showed that all obese patients had increased plasma triglyceride, cholesterol, LDL-cholesterol, -triglyceride and HDL-triglyceride levels concentrations compared to controls (P < 0.01). However, the younger obese men had relatively larger and accentuated changes in plasma lipids and lipoproteins than the older patients. Additionally, total antioxidant capacity (ORAC), vitamins C and E were lower while hydroperoxides and carbonyl proteins were higher in young and older obese patients compared to their respective controls (P < 0.001). Erythrocyte antioxidant SOD and catalase activities were enhanced in obese young patients, but reduced in obese older men. Glutathione peroxidase activity was low in obesity irrespective of age. In multiple regression analysis, BMI significantly predicted total cholesterol, LDL-C, LDL-TG and HDL-TG (P < 0.0001). These relationships were not modified by age. BMI alone was a not a significant predictor for ORAC, vitamins C, E, catalase and Glutathione peroxidase. However, the interaction BMI-age significantly predicted these parameters and explained 28-45% of their changes. BMI was a significant predictor of SOD, carbonyl proteins and hydroperoxides. This effect became more significant (P < 0.0001) and worsened with BMI-age interaction. In conclusion, lipoprotein metabolism and oxidant/antioxidant status are altered in obesity irrespective of age. However, obesity-related lipid and lipoprotein alterations were attenuated while oxidative stress was aggravated in older adults.
在这项研究中，我们研究了年轻（n = 45）和年老（n = 40）肥胖男性的血浆脂质、脂蛋白和氧化/抗氧化状态标志物，并将其与年轻（n = 65）和年老（n = 55）正常体重对照组进行了比较。目的是确定肥胖是否会加重老年男性的脂质、脂蛋白异常和氧化应激。我们的发现表明，所有肥胖患者的血浆甘油三酯、胆固醇、LDL-胆固醇、-甘油三酯和 HDL-甘油三酯水平均高于对照组（P < 0.01）。然而，年轻肥胖男性的血浆脂质和脂蛋白变化相对较大且更为明显。此外，与各自的对照组相比，年轻和年老肥胖患者的总抗氧化能力（ORAC）、维生素 C 和 E 水平较低，而氢过氧化物和羰基蛋白水平较高（P < 0.001）。肥胖的年轻患者的红细胞抗氧化 SOD 和过氧化氢酶活性增强，但肥胖的老年男性则降低。无论年龄大小，肥胖患者的谷胱甘肽过氧化物酶活性均较低。在多元回归分析中，BMI 显著预测总胆固醇、LDL-C、LDL-TG 和 HDL-TG（P < 0.0001）。这些关系不受年龄影响。BMI 本身并不是 ORAC、维生素 C、E、过氧化氢酶和谷胱甘肽过氧化物酶的重要预测因子。然而，BMI-年龄的相互作用显著预测了这些参数，并解释了它们变化的 28-45%。BMI 是 SOD、羰基蛋白和氢过氧化物的重要预测因子。这种影响变得更加显著（P < 0.0001），并且随着 BMI-年龄相互作用的加剧而恶化。总之，无论年龄大小，肥胖都会改变脂蛋白代谢和氧化/抗氧化状态。然而，肥胖相关的脂质和脂蛋白改变在老年人中减轻，而氧化应激加剧。
