Donadio S, Hutchinson C R
Corporate Molecular Biology, Abbott Laboratories, Abbott Park, IL 60064.
Gene. 1991 Apr;100:231-5. doi: 10.1016/0378-1119(91)90372-i.
The Saccharopolyspora erythraea gene (fdxA) corresponding to a previously purified ferredoxin [Shafiee and Hutchinson, J. Bacteriol., 170 (1988) 1548-1553] was cloned using an oligodeoxyribonucleotide probe based on the N-terminal sequence of the ferredoxin. The nucleotide sequence of a 1.3-kb segment encompassing fdxA indicates that the corresponding protein, SeFdI, is 105 amino acids long, and very similar to other 7Fe ferredoxins. A partial open reading frame closely linked to fdxA was also detected. Disruption of fdxA was attempted by replacing the wild-type allele with an in vitro mutated copy. The failure to construct an fdxA mutant strain suggests that fdxA lies in an essential region of the S. erythraea chromosome.
对应于先前纯化的铁氧化还原蛋白的糖多孢红霉菌基因（fdxA）[沙菲和哈钦森，《细菌学杂志》，170（1988）1548 - 1553]，使用基于该铁氧化还原蛋白N端序列的寡脱氧核糖核苷酸探针进行克隆。包含fdxA的1.3 kb片段的核苷酸序列表明，相应的蛋白质SeFdI长105个氨基酸，并且与其他7Fe铁氧化还原蛋白非常相似。还检测到一个与fdxA紧密相连的部分开放阅读框。试图通过用体外突变的拷贝替换野生型等位基因来破坏fdxA。未能构建出fdxA突变菌株表明fdxA位于糖多孢红霉菌染色体的一个必需区域。
