Arif Ibrahim A, Bakir Mohammad A, Khan Haseeb A, Al Farhan Ahmad H, Al Homaidan Ali A, Bahkali Ali H, Sadoon Mohammad Al, Shobrak Mohammad
Molecular Fingerprinting and Biodiversity Unit, Prince Sultan Research Chair for Environment and Wildlife, College of Sciences, King Saud University, Riyadh, Saudi Arabia.
Int J Mol Sci. 2010 May 10;11(5):2079-96. doi: 10.3390/ijms11052079.
Massive loss of valuable plant species in the past centuries and its adverse impact on environmental and socioeconomic values has triggered the conservation of plant resources. Appropriate identification and characterization of plant materials is essential for the successful conservation of plant resources and to ensure their sustainable use. Molecular tools developed in the past few years provide easy, less laborious means for assigning known and unknown plant taxa. These techniques answer many new evolutionary and taxonomic questions, which were not previously possible with only phenotypic methods. Molecular techniques such as DNA barcoding, random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), microsatellites and single nucleotide polymorphisms (SNP) have recently been used for plant diversity studies. Each technique has its own advantages and limitations. These techniques differ in their resolving power to detect genetic differences, type of data they generate and their applicability to particular taxonomic levels. This review presents a basic description of different molecular techniques that can be utilized for DNA fingerprinting and molecular diversity analysis of plant species.
在过去几个世纪中，大量珍贵植物物种的丧失及其对环境和社会经济价值的不利影响引发了对植物资源的保护。对植物材料进行恰当的识别和特征描述对于成功保护植物资源并确保其可持续利用至关重要。过去几年中开发的分子工具为鉴定已知和未知植物分类群提供了简便、省力的方法。这些技术回答了许多新的进化和分类学问题，而仅靠表型方法此前是无法做到的。诸如DNA条形码、随机扩增多态性DNA（RAPD）、扩增片段长度多态性（AFLP）、微卫星和单核苷酸多态性（SNP）等分子技术最近已用于植物多样性研究。每种技术都有其自身的优点和局限性。这些技术在检测遗传差异的分辨能力、所产生的数据类型以及对特定分类水平的适用性方面存在差异。本综述对可用于植物物种DNA指纹识别和分子多样性分析的不同分子技术进行了基本描述。
