Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea.
Med Mycol. 2011 Jan;49(1):1-9. doi: 10.3109/13693786.2010.492482. Epub 2010 Jun 21.
The formation of Candida biofilms has important clinical ramifications, because these biofilms exhibit increased resistance to conventional antifungal therapies. The aim of this study was to investigate the activity of xanthorrhizol on biofilms produced by non-C. albicans Candida (NCAC) species, including C. glabrata, C. guilliermondii, and C. parapsilosis. NCAC biofilms were generated in flat-bottom 96-well microtiter plates and quantified using the XTT (2, 3 - bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenyl amino) carbonyl]-2H-tetrazolium hydroxide) reduction assay. The NCAC biofilms at adherent, intermediate, and mature growth phases were treated with 0.5-512 μg/ml of xanthorrhizol for 24 h. The ranges of sessile minimum inhibitory concentrations (SMICs) of xanthorrhizol against C. glabrata, C. guilliermondii, and C. parapsilosis biofilms were 8-32 μg/ml, 8-16 μg/ml, and 8-64 μg/ml, respectively. Xanthorrhizol affected cell density that had an indirect effect on the biofilm OD(490). The compound eradicated the viable cells of the C. glabrata and C. parapsilosis biofilms at the adherent growth phase at 16 μg/ml and that of C. guilliermondii at 8 μg/ml. Treatment with 128 μg/ml of xanthorrhizol reduced the OD(490) of C. glabrata, C. guilliermondii, and C. parapsilosis biofilms at the mature growth phase by 77.8%, 88.5%, and 64.5%, respectively. These results indicate that xanthorrhizol exhibits potent activity against NCAC biofilms in vitro. Therefore, xanthorrhizol has potential therapeutic value in treating biofilm-associated NCAC infections and should be further evaluated in vivo.
念珠菌生物膜的形成具有重要的临床意义，因为这些生物膜对常规抗真菌治疗具有更高的耐药性。本研究旨在研究姜黄素对非白念珠菌念珠菌（NCAC）物种（包括近平滑念珠菌、光滑念珠菌和热带念珠菌）产生的生物膜的活性。NCAC 生物膜在平底 96 孔微量滴定板中生成，并通过 XTT（2,3-双（2-甲氧基-4-硝基-5-磺苯基）-5-[(苯氨基)羰基]-2H-四唑氢氧化铵）还原测定法进行定量。在附着、中间和成熟生长阶段，用 0.5-512 μg/ml 的姜黄素处理 NCAC 生物膜 24 h。姜黄素对近平滑念珠菌、光滑念珠菌和热带念珠菌生物膜的固着最小抑菌浓度（SMIC）范围分别为 8-32 μg/ml、8-16 μg/ml 和 8-64 μg/ml。姜黄素影响细胞密度，从而对生物膜 OD（490）产生间接影响。该化合物在 16 μg/ml 时可消除附着生长阶段的近平滑念珠菌和热带念珠菌生物膜的活细胞，在 8 μg/ml 时可消除光滑念珠菌生物膜的活细胞。用 128 μg/ml 的姜黄素处理可使成熟生长阶段的近平滑念珠菌、光滑念珠菌和热带念珠菌生物膜的 OD（490）分别降低 77.8%、88.5%和 64.5%。这些结果表明，姜黄素在体外对 NCAC 生物膜具有很强的活性。因此，姜黄素在治疗生物膜相关 NCAC 感染方面具有潜在的治疗价值，应在体内进一步评估。
