Laboratory of Natural Products-LHS, Department of Food Science, Faculty of Food Science and Technology, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor Darul Ehsan, Malaysia.
Phytother Res. 2013 Jul;27(7):1061-6. doi: 10.1002/ptr.4834. Epub 2012 Sep 12.
The purpose of this study was to investigate the activity of xanthorrhizol isolated from Curcuma xanthorrhiza Roxb. on Candida albicans biofilms at adherent, intermediate, and mature phase of growth. C. albicans biofilms were formed in flat-bottom 96-well microtiter plates. The biofilms of C. albicans at different phases of development were exposed to xanthorrhizol at different concentrations (0.5 µg/mL-256 µg/mL) for 24 h. The metabolic activity of cells within the biofilms was quantified using the XTT reduction assay. Sessile minimum inhibitory concentrations (SMICs) were determined at 50% and 80% reduction in the biofilm OD₄₉₀ compared to the control wells. The SMIC₅₀ and SMIC₈₀ of xanthorrhizol against 18 C. albicans biofilms were 4--16 µg/mL and 8--32 µg/mL, respectively. The results demonstrated that the activity of xanthorrhizol in reducing C. albicans biofilms OD₄₉₀ was dependent on the concentration and the phase of growth of biofilm. Xanthorrhizol at concentration of 8 µg/mL completely reduced in biofilm referring to XTT-colorimetric readings at adherent phase, whereas 32 µg/mL of xanthorrhizol reduced 87.95% and 67.48 % of biofilm referring to XTT-colorimetric readings at intermediate and mature phases, respectively. Xanthorrhizol displayed potent activity against C. albicans biofilms in vitro and therefore might have potential therapeutic implication for biofilm-associated candidal infections.
本研究旨在探讨姜黄醇自莪术（Curcuma xanthorrhiza Roxb.）对黏附、中期和成熟生长阶段白色念珠菌生物膜的活性。在平底 96 孔微量滴定板上形成白色念珠菌生物膜。将处于不同发育阶段的白色念珠菌生物膜暴露于不同浓度（0.5 µg/mL-256 µg/mL）的姜黄醇中 24 小时。使用 XTT 还原测定法定量测定生物膜内细胞的代谢活性。在与对照孔相比，生物膜 OD₅₀减少 50%和 80%时，确定固着最小抑菌浓度（SMIC）。姜黄醇对 18 株白色念珠菌生物膜的 SMIC₅₀和 SMIC₈₀分别为 4-16 µg/mL 和 8-32 µg/mL。结果表明，姜黄醇降低白色念珠菌生物膜 OD₅₀的活性取决于生物膜浓度和生长阶段。浓度为 8 µg/mL 的姜黄醇在黏附阶段的 XTT 比色读数完全减少了生物膜，而 32 µg/mL 的姜黄醇在中间和成熟阶段的 XTT 比色读数分别减少了 87.95%和 67.48%的生物膜。姜黄醇对体外白色念珠菌生物膜具有很强的活性，因此可能对生物膜相关念珠菌感染具有潜在的治疗意义。
