Department of Obstetrics and Gynecology, Wayne State University, Detroit, MI, USA.
Am J Reprod Immunol. 2010 Aug 1;64(2):137-51. doi: 10.1111/j.1600-0897.2010.00881.x. Epub 2010 Jun 15.
Emerging evidence suggests that metabolism influences immune cell signaling and immunoregulation. To examine the immunoregulatory role of glycolysis in pregnancy, we evaluated the properties of pyruvate kinase in leukocytes from non-pregnant women and those with normal pregnancy and pre-eclampsia.
We evaluated pyruvate kinase expression in lymphocytes and neutrophils from non-pregnant, pregnant, and pre-eclampsia patients using fluorescence microscopy and flow cytometry. Leukocyte pyruvate kinase activity and pyruvate concentrations were also evaluated. To study pyruvate's effect on signaling, we labeled Jurkat T cells with Ca(2+) dyes and measured cell responses in the presence of agents influencing intracellular pyruvate.
The expression of pyruvate kinase is reduced in lymphocytes and neutrophils from normal pregnant women in comparison with those of non-pregnant women and pre-eclampsia patients. Similarly, the activity of pyruvate kinase and the intracellular pyruvate concentration are reduced in leukocytes of normal pregnant women in comparison with non-pregnant women and women with pre-eclampsia. Using Jurkat cells as a model of leukocyte signaling, we have shown that perturbations of intracellular pyruvate influence Ca(2+) signals.
Normal pregnancy is characterized by reduced pyruvate kinase expression within lymphocytes and neutrophils. We speculate that reduced pyruvate kinase expression modifies immune cell responses due to reduced pyruvate concentrations.
新出现的证据表明，代谢会影响免疫细胞信号和免疫调节。为了研究糖酵解在妊娠中的免疫调节作用，我们评估了非孕妇、正常妊娠和子痫前期患者白细胞中丙酮酸激酶的特性。
我们使用荧光显微镜和流式细胞术评估了非孕妇、孕妇和子痫前期患者淋巴细胞和中性粒细胞中丙酮酸激酶的表达。还评估了白细胞丙酮酸激酶活性和丙酮酸浓度。为了研究丙酮酸对信号的影响，我们用 Ca(2+)染料标记 Jurkat T 细胞，并在影响细胞内丙酮酸的试剂存在的情况下测量细胞反应。
与非孕妇和子痫前期患者相比，正常妊娠妇女的淋巴细胞和中性粒细胞中丙酮酸激酶的表达降低。同样，与非孕妇和子痫前期患者相比，正常妊娠妇女的白细胞中丙酮酸激酶的活性和细胞内丙酮酸浓度降低。我们使用 Jurkat 细胞作为白细胞信号的模型，表明细胞内丙酮酸的干扰会影响 Ca(2+)信号。
正常妊娠的特点是淋巴细胞和中性粒细胞中丙酮酸激酶的表达降低。我们推测，由于丙酮酸浓度降低，丙酮酸激酶表达降低会改变免疫细胞的反应。
