Wu Zhen, Gao Ruiping, Li Hong, Wang Yongde, Luo Yang, Zou Jiang, Zhao Bo, Chen Shiqi
Chongqing Key Laboratory of Chinese Medicine & Health Science, Chongqing Academy of Chinese Materia Medica Chongqing 400065 P. R. China
College of Environment and Resources, Chongqing Technology and Business University Chongqing 400067 P. R. China.
RSC Adv. 2021 Oct 8;11(53):33219-33234. doi: 10.1039/d1ra03640h.
The combinatorial use of dietary jujube () and ginger play a critical role in traditional Chinese medicines, folk medicine and dietary therapy. Joint effects were investigated from the viewpoint of the antioxidant (scavenging DPPH˙) and antitumor activities (against SW620 cells) of jujube polysaccharides and ginger 6-gingerol (G6G) alone and in combination. Jujube polysaccharides were extracted, purified, and characterized, and then their inhibiting and apoptotic effects alone and in combination with G6G were evaluated by the cytological tests, including Cell Counting Kit-8, colony-forming, Annexin V-FITC and propidium iodide, TdT-mediated dUTP nick end labeling (TUNEL) staining, and cell cycle assays. Results showed that the purified polysaccharide fraction (ZJPs-II) with average molecular weight of 115 kDa consisted of arabinose, rhamnose, glucose, xylose, and galactose. ZJPs-II and G6G alone dose-dependently scavenged DPPH˙ and inhibited the proliferation of SW620 cells, while their combination showed synergistic interactions (all combination index < 1). The studies further demonstrated that ZJPs-II and G6G alone reduced the cell colony-formation, induced apoptosis and arrested the cell-cycle at G2/M phase, while their combination achieved better effects and significantly arrested the growth at the G0/G1 phase. Collectively, our findings suggest enhancing the intake of jujube polysaccharides and G6G in a combinatorial approach for maintaining health and preventing cancer.
在传统中药、民间医药和食疗中，食用枣和姜的组合发挥着关键作用。从枣多糖和姜辣素（G6G）单独及联合使用的抗氧化（清除DPPH˙）和抗肿瘤活性（针对SW620细胞）的角度研究了联合效应。提取、纯化并表征了枣多糖，然后通过细胞计数试剂盒-8、集落形成、膜联蛋白V-异硫氰酸荧光素和碘化丙啶、末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TUNEL）染色以及细胞周期分析等细胞学试验评估了它们单独及与G6G联合使用时的抑制和凋亡作用。结果表明，平均分子量为115 kDa的纯化多糖组分（ZJPs-II）由阿拉伯糖、鼠李糖、葡萄糖、木糖和半乳糖组成。ZJPs-II和G6G单独使用时均呈剂量依赖性清除DPPH˙并抑制SW620细胞的增殖，而它们的组合显示出协同相互作用（所有组合指数<1）。研究进一步表明，ZJPs-II和G6G单独使用时均可减少细胞集落形成、诱导凋亡并使细胞周期停滞在G2/M期，而它们的组合效果更佳，并显著使生长停滞在G0/G1期。总体而言，我们的研究结果表明，以组合方式增加枣多糖和G6G的摄入量有助于维持健康和预防癌症。
