突尼斯牛至（Origanum vulgare L. subsp. glandulosum （Desf.） Ietswaart）精油的化学成分和抗氧化活性。

Essential oils of Origanum vulgare L. subsp. glandulosum (Desf.) Ietswaart from Tunisia: chemical composition and antioxidant activity.

机构信息

Unité de Biochimie des lipides et des protéines, Faculté des Sciences de Tunis, Campus Universitaire EL Manar, Tunis 2092, Tunisia.

出版信息

J Sci Food Agric. 2010 Aug 15;90(10):1745-9. doi: 10.1002/jsfa.4011.

DOI:10.1002/jsfa.4011

PMID:20564436

Abstract

BACKGROUND

Characterisation of the essential oils from O. glandulosum collected in three locations of Tunisia, chemical composition and the evaluation of their antioxidant activities were carried out.

RESULTS

The essential oils from Origanum vulgare L. subsp. glandulosum (Desf.) Ietswaart collected from three localities of north Tunisia-Krib, Bargou and Nefza-were obtained in yields of 2.5, 3.0 and 4.6% (v/w), respectively. The essential oils were analysed by GC and GC/MS and assayed for their total phenolics content, by the Folin-Ciocalteu method, and antioxidant effectiveness, using the 2,2-diphenyl-1-picrylhydrazil (DPPH) radical scavenging assay. The main components of these essential oils, from Nefza, Bargou and Krib, were p-cymene (36%, 40% and 46%), thymol (32%, 39% and 18%), gamma-terpinene (24%, 12% and 16%) and carvacrol (2%, 2% and 15%), respectively). The ability to scavenge the DPPH radicals, expressed by IC(50), ranged from 59 to 80 mg L(-1). The total phenolic content, expressed in gallic acid equivalent (GAE) g kg(-1) dry weight, varied from 9.37 to 17.70 g kg(-1) dw.

CONCLUSIONS

A correlation was identified between the total phenolic content of the essential oils and DPPH radical scavenger capacity. The occurrence of a p-cymene chemotype of O. glandulosum in the northern region of Tunisia is demonstrated.

摘要

背景

对在突尼斯三个地区采集的牛至 O. glandulosum 的精油进行了特征描述、化学成分分析和抗氧化活性评估。

结果

从突尼斯北部的三个地区（克里布、巴尔古和内夫扎）采集的牛至 O. vulgare L. subsp. glandulosum（Desf.）Ietswaart 的精油产率分别为 2.5%、3.0%和 4.6%（v/w）。通过 GC 和 GC/MS 对精油进行了分析，并采用 Folin-Ciocalteu 法测定了总酚含量，采用 2,2-二苯基-1-苦基肼（DPPH）自由基清除法测定了抗氧化活性。这些精油的主要成分，来自内夫扎、巴尔古和克里布，分别为对伞花烃（36%、40%和 46%）、百里酚（32%、39%和 18%）、γ-萜品烯（24%、12%和 16%）和香芹酚（2%、2%和 15%）。清除 DPPH 自由基的能力，以 IC50 表示，范围为 59 至 80mg L-1。总酚含量，以没食子酸当量（GAE）g kg-1 干重表示，范围为 9.37 至 17.70 g kg-1 dw。