Kohat Institute of Medical Sciences, Kohat University of Science and Technology, Kohat, N.W.F.P., Pakistan.
J Enzyme Inhib Med Chem. 2010 Dec;25(6):773-8. doi: 10.3109/14756360903433365. Epub 2010 Jun 22.
The CHCl(3)-soluble fraction of the whole plant of Duranta repens showed anti-plasmodial activity against the chloroquine-sensitive (D6) and chloroquine-resistant (W2) strains of Plasmodium falciparum, with IC(50) values of 8.5 ± 0.9 and 10.2 ± 1.5 μg/mL, respectively. From this fraction, two new flavonoid glycosides, 7-O-α-d-glucopyranosyl-3,4'-dihydroxy-3'-(4-hydroxy-3-methylbutyl)-5,6-dimethoxyflavone (1) and 7-O-α-d-glucopyranosyl(6'''-p-hydroxcinnamoyl)-3,4'-dihydroxy-3'-(4-hydroxy-3-methylbutyl)-5,6-dimethoxyflavone (2), along with five known flavonoids, 3,7,4'-trihydroxy-3'-(4-hydroxy-3-methylbutyl)-5,6-dimethoxyflavone (3), 3,7-dihydroxy-3'-(4-hydroxy-3-methylbutyl)-5,6,4'-trimethoxyflavone (4), 5,7-dihydroxy-3'-(2-hydroxy-3-methyl-3-butenyl)-3,6,4'-trimethoxyflavone (5), 3,7-dihydroxy-3'-(2-hydroxy-3-methyl-3-buten-yl)-5,6,4'-trimethoxyflavone (6), and 7-O-α-d-glucopyranosyl-3,5-dihydroxy-3'-(4''-acetoxy-3''-methylbutyl)-6,4'-dimethoxyflavone (7), have been isolated as anti-plasmodial principles. Their structures were deduced by spectroscopic analysis including 1D and 2D NMR techniques. The compounds (1-7) showed potent anti-plasmodial activities against D6 and W2 strains of Plasmodium falciparum, with IC(50) values in the range of 5.2-13.5 μM and 5.9-13.1 μM, respectively.
苦丁菜全草的 CHCl3 可溶部分对氯喹敏感（D6）和氯喹耐药（W2）株疟原虫表现出抗疟活性，IC50 值分别为 8.5±0.9 和 10.2±1.5μg/mL。从该部分分离得到两种新的黄酮苷，7-O-α-d-吡喃葡萄糖基-3,4'-二羟基-3'-(4-羟基-3-甲基丁基）-5,6-二甲氧基黄酮（1）和 7-O-α-d-吡喃葡萄糖基（6'''-对羟基肉桂酰基）-3,4'-二羟基-3'-(4-羟基-3-甲基丁基）-5,6-二甲氧基黄酮（2），以及五种已知的黄酮类化合物，3,7,4'-三羟基-3'-(4-羟基-3-甲基丁基）-5,6-二甲氧基黄酮（3），3,7-二羟基-3'-(4-羟基-3-甲基丁基）-5,6,4'-三甲氧基黄酮（4），5,7-二羟基-3'-(2-羟基-3-甲基-3-丁烯基）-3,6,4'-三甲氧基黄酮（5），3,7-二羟基-3'-(2-羟基-3-甲基-3-丁烯基）-5,6,4'-三甲氧基黄酮（6）和 7-O-α-d-吡喃葡萄糖基-3,5-二羟基-3'-(4''-乙酰氧基-3''-甲基丁基）-6,4'-二甲氧基黄酮（7），已被分离为抗疟原虫的原则。它们的结构是通过包括 1D 和 2D NMR 技术在内的光谱分析推断出来的。化合物（1-7）对 D6 和 W2 株疟原虫表现出很强的抗疟活性，IC50 值在 5.2-13.5μM 和 5.9-13.1μM 范围内。
