Donkor Shadrack, Larbie Christopher, Komlaga Gustav, Emikpe Benjamin Obukowho
Applied Radiation Biology Centre, Radiological and Medical Sciences Research Institute, Ghana Atomic Energy Commission, Legon, Accra, Ghana.
Department of Biochemistry and Biotechnology, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana.
J Toxicol. 2020 Dec 9;2020:8815719. doi: 10.1155/2020/8815719. eCollection 2020.
Liver disease is a major health problem and its treatment is costly in most developing countries with attendant adverse effects. This study aimed at determining the acute hepatoprotective efficacy of hydroethanolic extracts of leaves, ripe and unripe fruits against CCl-, and acetaminophen-induced hepatotoxicity in animals. . CCl (1 mL/kg body weight in olive oil) and acetaminophen (500 mg/kg b.wt) were used to induce hepatotoxicity in the animals. Animals were treated with extracts at 250 mg/kg b.wt and standard drug, silymarin (100 mg/kg), for 7 days. Hepatoprotective efficacy was assessed by assaying serum biochemical markers such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma-glutamyl transferase (GT), bilirubin (Bil), antioxidative biomarkers including reduced glutathione (GSH), glutathione peroxidase (GPx), glutathione transferase (GST), superoxide dismutase (SOD), malondialdehyde (MDA), hydrogen peroxidase (H0), and nitric oxide (NO), as well as histological observations. . Exposure of the animals to CCl and acetaminophen resulted in liver injury as evidenced by elevated ALT, AST, ALP, GT, Bil, MDA, HO, and NO levels with resultant derangement in liver microarchitecture. Pretreatment with hydroethanolic extracts, particularly ripe fruits of , led to a reduction in these indicators and an increase in GSH, GPx, GST, and SOD levels. Biochemical data were supported by improvement in liver structure. . The findings suggest that hydroethanolic ripe fruits extract possesses hepatoprotective and antioxidative activities against CCl- and acetaminophen-induced toxicity and could be developed as a potent agent for drug-induced liver diseases.
在大多数发展中国家，肝脏疾病是一个重大的健康问题，其治疗成本高昂且伴有不良影响。本研究旨在确定叶、成熟果实和未成熟果实的水乙醇提取物对动物四氯化碳和对乙酰氨基酚诱导的肝毒性的急性肝保护功效。用四氯化碳（1毫升/千克体重溶于橄榄油）和对乙酰氨基酚（500毫克/千克体重）诱导动物肝毒性。动物分别用250毫克/千克体重的提取物和标准药物水飞蓟宾（100毫克/千克）治疗7天。通过检测血清生化标志物如丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、碱性磷酸酶（ALP）、γ-谷氨酰转移酶（GT）、胆红素（Bil）、抗氧化生物标志物包括还原型谷胱甘肽（GSH）、谷胱甘肽过氧化物酶（GPx）、谷胱甘肽转移酶（GST）、超氧化物歧化酶（SOD）、丙二醛（MDA）、过氧化氢酶（H0）和一氧化氮（NO）以及组织学观察来评估肝保护功效。动物接触四氯化碳和对乙酰氨基酚导致肝损伤，表现为ALT、AST、ALP、GT、Bil、MDA、HO和NO水平升高，肝脏微结构紊乱。用水乙醇提取物预处理，特别是[植物名称]的成熟果实提取物，可使这些指标降低，GSH、GPx、GST和SOD水平升高。肝脏结构的改善支持了生化数据。研究结果表明，水乙醇成熟果实提取物对四氯化碳和对乙酰氨基酚诱导的毒性具有肝保护和抗氧化活性，可以开发成为治疗药物性肝病的有效药物。
