Evaluation of the Acute Hepatoprotective Potential of Hydroethanolic Extract of L. Parts.

Liver disease is a major health problem and its treatment is costly in most developing countries with attendant adverse effects. This study aimed at determining the acute hepatoprotective efficacy of hydroethanolic extracts of leaves, ripe and unripe fruits against CCl-, and acetaminophen-induced hepatotoxicity in animals. . CCl (1 mL/kg body weight in olive oil) and acetaminophen (500 mg/kg b.wt) were used to induce hepatotoxicity in the animals. Animals were treated with extracts at 250 mg/kg b.wt and standard drug, silymarin (100 mg/kg), for 7 days. Hepatoprotective efficacy was assessed by assaying serum biochemical markers such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma-glutamyl transferase (GT), bilirubin (Bil), antioxidative biomarkers including reduced glutathione (GSH), glutathione peroxidase (GPx), glutathione transferase (GST), superoxide dismutase (SOD), malondialdehyde (MDA), hydrogen peroxidase (H0), and nitric oxide (NO), as well as histological observations. . Exposure of the animals to CCl and acetaminophen resulted in liver injury as evidenced by elevated ALT, AST, ALP, GT, Bil, MDA, HO, and NO levels with resultant derangement in liver microarchitecture. Pretreatment with hydroethanolic extracts, particularly ripe fruits of , led to a reduction in these indicators and an increase in GSH, GPx, GST, and SOD levels. Biochemical data were supported by improvement in liver structure. . The findings suggest that hydroethanolic ripe fruits extract possesses hepatoprotective and antioxidative activities against CCl- and acetaminophen-induced toxicity and could be developed as a potent agent for drug-induced liver diseases.