Khan Md Asaduzzaman, Rahman Mohammad Mijanur, Tania Mousumi, Shoshee Nusrat Fatima, Xu Ai-hua, Chen Han-chun
Department of Biochemistry, School of Biological Science and Technology, Central South University, Changsha, Hunan 410013, China.
Afr J Tradit Complement Altern Med. 2013 Apr 12;10(3):436-41. eCollection 2013.
The effects of Duranta repens fruits were investigated on H2O2 induced oxidative cell death to evaluate its antioxidative potential in vitro. HEK293T cells were treated with different concentrations [0-1000 µg/ ml] of ethanol extract (E-Ex) and methanol extract (M-Ex) of D. repens for 24h, and then treated with 100 µM H2O2 for 24h. Cell viability, antioxidant parameters of cells, and antioxidant constituents of the extracts were determined. Treatment with limited dose of E-Ex or M-Ex increased the survival rate of H2O2-treated HEK293T cells, however the extra-high dose showed growth inhibitory effect. Treatment with E-Ex or M-Ex protected cellular lipid per-oxidation. In vitro analyses showed the 2,2-diphenyl-1-picrylhydrazyl and H2O2 scavenging activities as well as reducing potential of the extracts. We report here that the limited dose of E-Ex and M-Ex possess antioxidative potential, which can protect H2O2-induced oxidative cell damage.
研究了黄金叶果实对过氧化氢诱导的细胞氧化死亡的影响，以评估其体外抗氧化潜力。将人胚肾细胞293T（HEK293T）用不同浓度[0 - 1000微克/毫升]的黄金叶乙醇提取物（E-Ex）和甲醇提取物（M-Ex）处理24小时，然后用100微摩尔/升过氧化氢处理24小时。测定细胞活力、细胞的抗氧化参数以及提取物的抗氧化成分。用有限剂量的E-Ex或M-Ex处理可提高过氧化氢处理的HEK293T细胞的存活率，但超高剂量则显示出生长抑制作用。用E-Ex或M-Ex处理可保护细胞脂质过氧化。体外分析显示了提取物的2,2-二苯基-1-苦基肼自由基清除活性、过氧化氢清除活性以及还原能力。我们在此报告，有限剂量的E-Ex和M-Ex具有抗氧化潜力，可保护过氧化氢诱导的细胞氧化损伤。
