Khanal Pukar, Patil Basanagouda M
Department of Pharmacology and Toxicology, KLE College of Pharmacy, Belagavi, KLE Academy of Higher Education and Research (KAHER), Belagavi 590010, India.
Chin Herb Med. 2020 Aug 19;12(4):406-413. doi: 10.1016/j.chmed.2020.02.004. eCollection 2020 Oct.
To report anti-oxidant activity and cytotoxicity of hydroalcoholic extract of (bark) and (whole plant), and present the probable biological spectrum of major anti-oxidants from both plants.
The coarse powder of both plants was first extracted with 70% ethanol (maceration) followed by 99% ethanol (Soxhlet-extraction). Anti-oxidant activity of the extracts was evaluated using DPPH, HO, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS), NO scavenging assay, total antioxidant capacity, cupric reducing antioxidant capacity (CUPRAC), and metal chelating assay. Cytotoxicity of both extracts was evaluated using MTT assay in both tumor and normal cell lines i.e. Chinese hamster ovary cells (CHO) and A549 cells. Biological activity of individual anti-oxidants from both medicinal plants was identified using prediction of activity spectra for substances and a docking study was performed by using autodock4.0.
Hydroalcoholic extract of and showed the highest free radical scavenging (ABTS) and chelating capacity respectively. Both extracts showed minimum cytotoxicity in normal cell lines compared to tumor cell lines. Computer imitation hits reflected the multiple biological activities agreeing with the folk use and some scientific reports. Further, we found the binding affinity of predicted anti-oxidant compounds with multiple protein molecules involved in oxidative stress.
The present study reports the probable anti-oxidant mechanism for two folk agents and also presents probable pharmacological activities via computer simulations.
报告[植物名称1]（树皮）和[植物名称2]（全株）水醇提取物的抗氧化活性和细胞毒性，并展示这两种植物主要抗氧化剂可能的生物活性谱。
首先用70%乙醇对两种植物的粗粉进行浸渍提取，然后用99%乙醇进行索氏提取。使用DPPH、羟基自由基（HO）、2,2'-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）（ABTS）、一氧化氮清除试验、总抗氧化能力、铜离子还原抗氧化能力（CUPRAC）和金属螯合试验评估提取物的抗氧化活性。使用MTT试验在肿瘤细胞系和正常细胞系即中国仓鼠卵巢细胞（CHO）和A549细胞中评估两种提取物的细胞毒性。使用物质活性谱预测确定两种药用植物中单个抗氧化剂的生物活性，并使用autodock4.0进行对接研究。
[植物名称1]和[植物名称2]的水醇提取物分别表现出最高的自由基清除（ABTS）和螯合能力。与肿瘤细胞系相比，两种提取物在正常细胞系中显示出最小的细胞毒性。计算机模拟结果反映了多种生物活性，这与民间用途和一些科学报告一致。此外，我们发现预测的抗氧化化合物与参与氧化应激的多种蛋白质分子具有结合亲和力。
本研究报告了两种民间用药可能的抗氧化机制，并通过计算机模拟展示了可能的药理活性。
