IBP, Bat 630, Université Paris Sud, Orsay 91405, France.
Mol Plant Pathol. 2004 May 1;5(3):203-16. doi: 10.1111/j.1364-3703.2004.00219.x.
SUMMARY The infection of plants by pathogenic microbes and the subsequent establishment of disease involves substantial changes in the biochemistry and physiology of both partners. Analysis of genes that are expressed during these interactions represents a powerful strategy to obtain insights into the molecular events underlying these changes. Root diseases have considerable economic impact but have not been characterized extensively at the molecular genetic level. Here we have used two complementary approaches-suppression subtractive hybridization and expressed sequence tag analysis of an unsubtracted cDNA library-to investigate gene expression during the early stages of colonization of wheat roots by the take-all fungus, Gaeumannomyces graminis.
植物受到病原微生物的感染以及随后疾病的发生，涉及到双方生物化学和生理学的巨大变化。对这些相互作用过程中表达的基因进行分析，是深入了解这些变化所涉及的分子事件的有力策略。根部病害对经济有重大影响，但在分子遗传学水平上还没有得到广泛的描述。在这里，我们使用了两种互补的方法——抑制差减杂交和未消减 cDNA 文库的表达序列标签分析——来研究小麦根被禾顶囊壳菌侵染的早期阶段的基因表达。
